Organic acids peak at similar RT using HPLC?

My colleague doing the organic acid detection using pelkin almer HPLC unit, RP-HPLC, UV detector. But she got difficulties because some of the organic acid peak were existed at similar RT...

29 December 2020 2,843 5 View

Does society influence on language or language influence on society? How?

The public question is to all experts of linguists, applied linguists and sociolinguists. This helps to figure out the impact of society in language and the impact of language in society.

28 June 2020 3,048 3 View

How to calculate the sample size in muscle assessment studies?

We usually take the sample size from previous literature while working on muscle function assessment using some non-invasive technique like electromyography or mechanomyography. Any one knows...

06 February 2019 6,425 2 View

In disinfection process, how can a certain disinfectant target only pathogenic microorganism?

I understand that disinfection is to eliminate most harmful microorganisms from objects; while sterilization is to to kill ALL microbes - whether harmful or not. I wonder how can a certain...

14 January 2019 1,836 3 View

Is artemether soluble in acetic acid?

Please explain the molarity of the acetic acid for solubilization of artemether.

22 September 2018 4,728 3 View

Ground water model in agricultural watershed to study the fluctuation in groundwater table because of water withdrawals from wells?

16 May 2017 6,944 9 View

Can we obtain antibody against certain toxin in bacteria and how ?

07 August 2016 7,982 6 View

Lib-SVM Classifier technical question:

22 April 2016 907 2 View

What is the difference between Classification Map and Taxonomy Map

I would like to do a systematic review for the literature review but i found 2 maps. 1- Classification Map. 2- Taxonomy Map. What are the differences between them in term of number of papers that...

01 January 1970 9,282 12 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

How to show in a scientific paper format the post-hoc test of 4 variables involved, when 3 by 3 variables are significant?

In a study involving 4 variables (species(2x), temperature(2x), nutrients(2x) and light(3x)), 3 by 3 variables, for example, "species", "temperature" and "nutrients" are significant. I end up with...

15 February 2021 8,018 2 View