Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

How do I know if my GLM using selection of models by Akaike Information Criterion is ok?

I'm trying to see the isolated and combined effects of precipitation and three different human disturbances indexes are having an effect on the growth of a cactus. I have a gradient of eight...

16 May 2020 5,868 5 View

How to interpret TGA degradation temperature shifts in encapsulated essential oil?

Hi, I am encapsulating essential oils in silica mesoporous nanocapsules. I performed a TGA just with the essential oil and another one for the silica nanocapsules loaded with the oil. I identified...

23 April 2020 3,069 1 View

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I took four slides out from -80 freezer. Then I realized one of the reagents was over but I couldn't see it before because the container is dark. What can I do? Do another staining or can I...

25 July 2017 7,733 3 View

Start programming in Julia?

I want to start programming with Julia, what would be a good starting point? Any book, online free course or recommendation?

05 June 2017 7,781 2 View

Has anyone worked on physical behavior of wood with comsol or ansys?

I seek to share information... Thanks

10 November 2016 3,811 0 View

We are interested in electronic devices that measure the diameter of the trees daily and communicate with data logger. What are the best devices?

09 October 2015 6,788 6 View

Does someone know the range of malondialdehyde of a plant of rice under heat stress?

I'm working with high night temperature in rice. In some articles I find a range of 20-200 nmol g-1 fw and others a range of 2-50 nmol g-1 fw. Are these ranges correct?

19 March 2014 5,109 3 View

How to interpret an absortion peak around 220 nm in a silver nanoparticle solution?

I've synthesized silver nanoparticles via sonochemistry. In addition to the well known peak around 400 nm, I've observed another one around 220 nm which do not correspond to any of the reagents...

17 June 2013 9,142 27 View

Which assay at plant level is better for screening if bacteria have plant growth promotion activity?

Plant species? Conditions?

16 May 2013 9,294 5 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

Can Immunogenic BNT162b vaccines protect HUMANS from SARS-CoV-2?

"A safe and effective vaccine against COVID-19 is urgently needed in quantities sufficient to immunise large populations. We report the preclinical development of two BNT162b vaccine candidates,...

11 February 2021 6,628 9 View