How can we measure the energy quality (stability, duration, peaks, etc.) for commercial / house uses?
I'm a Venezuelan PE Teacher working in a British International School at Caracas, Venezuela and one of my Appraisal Projects is to Adapt the UK National Curriculum to the Venezuelan Context in...
01 November 2023 7,149 0 View
I am separating cellular fractions of liver tissues for western blot. So far I have been successful with my cytosolic and mitochondrial fractions but struggling to increase the purity of my...
25 January 2023 7,530 1 View
I'm using a glucose colorimetric detection kit from Thermo (EIAGLUC), but I always get a lot of intra and inter-assay variability, sometimes even between replicates. I'm measuring glucose in...
22 June 2022 3,278 3 View
Would 10 minutes of incubation with glucose and insulin be enough to measure changes in glucose concentration in culture media using adipocytes? I use a glucose oxidase colorimetric kit. Thank...
13 January 2022 3,533 1 View
Good afternoon. Recently in my lab, we bought a Shimadzu HPLC and I have a question if someone have used this equipment. How much time do you equilibrated your HPLC before the run of your samples?
31 May 2021 6,634 4 View
Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...
18 February 2021 3,203 4 View
I'm trying to see the isolated and combined effects of precipitation and three different human disturbances indexes are having an effect on the growth of a cactus. I have a gradient of eight...
16 May 2020 9,778 13 View
16 May 2020 6,105 5 View
Hi, I am encapsulating essential oils in silica mesoporous nanocapsules. I performed a TGA just with the essential oil and another one for the silica nanocapsules loaded with the oil. I identified...
23 April 2020 3,301 1 View
We are looking for diverse approaches to disassociating fin tissues from a fish. We are currently using TrypLE but we've got a feeling that spiking it with additional proteases may get the job...
09 March 2020 8,368 3 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!
29 July 2024 488 0 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...
23 July 2024 9,109 0 View
Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...
22 July 2024 8,770 1 View
I need to know the approximate size of my target protein for Negative-stain EM. I have an alpha-fold predicted structure of the protein. I do not see any turtorial on internet for estimating the...
21 July 2024 5,732 3 View
Research being conducted by Stellenbosch University would like your opinion about the effects of social exclusion on emerging urban mobility options and traditional public transport services. By...
21 July 2024 4,032 0 View
I do not know much about FTIR! The material Benzethonium Chloride USP was compared to a reference standard in regards to FTIR, as per the USP testing: Identification B. Infrared absorption (FTIR),...
16 July 2024 6,918 7 View