Hi! can i do western blot for foxp3 protein in intestinal biopsy sample? how?

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Dmitry Zinovkin

Good day, Nastaran Asri

FoxP3 expressed mostly Treg and in mucosa glands of GI tract it not expressed and Human Protein Atlas could help you

Here is links to Foxp3 in it https://www.proteinatlas.org/ENSG00000049768-FOXP3/cell

https://www.proteinatlas.org/ENSG00000049768-FOXP3/tissue

Nastaran Asri

Dmitry Zinovkin thank you so much.:)

Claudio Pioli

Why not to try RT-PCR to detect Foxp3 mRNA? I'm supposing you have small samples; the majority of the cells are likely not to express Foxp3