I have varied the glyphosate concentration to derivatize with 20 nM of FMOC-Cl but i am not getting linearity for the derivatives for my calibration curve. I used UHPLC-ToF-MS/S, negative polarity for my analysis .
Hi Raymond,
you have to use a surplus of FMOC-Cl to get a quantitative conversion. Otherwise you have the effect that your calibration function shows no linear course. Excess FMOC-Cl can be intercepted with aminoadamantane.
Look here: (page 1198 is important)
Article A Simple and Efficient Method for Derivatization of Glyphosa...
Regards
Markus
Thank you Markus. I used 20 mM of FMOC which is over a 5000 ppm. Is this not enough from your experience?@Markus Christ
Hi Raymond,
the authors of the article above wrote: "The stoichiometric ratio between the analyte and the derivatizing reagent affects derivatized product formation. Previous studies indicated that a 1:1 ratio was ineffective for the reaction. Thus, this study used 200 and 500 mg/L FMOC-Cl, representing the proportions of 1:2 and 1:5 for the analyte and FMOC-Cl, respectively."
What is your ratio of analyte and FMOC-Cl?
Regards
Markus
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