Hi All, I am going to work with glycine receptors in CHO cells. Could anyone recommend me the best and not expensive way to do a fast application?

23 January 2018 0 7K Report

I checked the Warner Instruments. They have a fast-step perfusion system. Could you give me some tips how to do the fast application for low price. Thank you!

Badges
Science topic

More Iuliia Polina's questions See All

How to prepare a molecule for molecular dynamics calculations?

If I want to calculate molecular dynamics (Gromacs), do I first need to optimize the geometry of the molecule? For example, I first draw the molecule in Avogadro, and then what? I guess I can't...

28 July 2024 673 3 View

Why Orca calculations take too loong?

Hello! I am trying to calculate the sumatriptan molecule, but it has been counting for five days and it does not stop. It seems to me that it is too long and I am doing something wrong. Please...

25 July 2024 3,364 5 View

ORCA. What does this error mean: cleanup molecule is Linear but not aligned to z-axis?

Dear researchers, Here is my input file: # avogadro generated ORCA input file # Basic Mode # ! RHF SP def2-SVP Opt Freq MORead #! BP RI OPT FREQ def2-SVP def2/J MORead %moinp "co2.gbw" *...

12 June 2024 1,810 6 View

Does it necessary to perform BCA analysis for doing ELISA or dot-blot with serum?

Hi everyone! I am used to doing BCA test while doing protein extraction from cells or tissue, but do you usually use it while working with serum, i.e. mouse serum? Or do you just consider your...

23 March 2024 4,437 2 View

What strategy can Tesla use to improve their brand image and consumer trust as the most innovative car company?

The question is for a business case research plan where a theory and additional research is needed.

22 March 2024 7,263 1 View

How to increase the amount of memory in Workspace when modeling in real time in Matlab?

Data is recorded only for 20 seconds of the experiment with a sampling time of 0.002 s. (Matlab v. 2015)

07 January 2024 2,240 2 View

CST Studio: How to perform temperature dependence of dielectric permittivity and conductivity?

Greetings! I have a task to estimate microwave heating of dielectric object vs time. For this reason I need to make dielectric permittivity and conductivity of material under investigation...

06 October 2023 3,077 2 View

What are the consciousness states where one would see an absolute power decrease in the EEG?

I am making a comparison between different states of consciousness and I would like to ask here to make sure I have not missed anything important.

09 August 2022 5,570 3 View

Are you aware of any sources of information describing laboratory experiments on the interaction of CO2 and rocks or seals?

I am currently working on a project to evaluate CO2 storage sites where one of the interests is there a chemical interaction between the rock, the seal and the injected CO2 agent?

07 December 2021 265 1 View

Which group psychotherapy questionnaire?

Hello, My team has planned to diagnostically follow one psychotherapy group with questionnaires. I wanted to use the TFI (therapeutic factors inventory) to analyze therapeutic factors based on...

20 November 2021 301 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How effective is the Citi Bloc standard basket in enhancing the accuracy and comparability of international construction cost assessments?

Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...

04 August 2024 8,997 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How combine yolo with Faster R-CNN?

I want a model that is balanced with accuracy or speed, faster rcnn has high accuracy while yolo have fast speed. i am thinking to combine them to get a hybrid model to achieve both speed and accuracy

02 August 2024 3,104 0 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View