What is the best protocol to extract bacterial genomic DNA from cheese?

Hello everyone, Actually I am trying to extract bacterial genomic DNA from cheese. I am using isolate II genomic DNA kit. Sometimes it gives good results but sometimes I don't get anything. This...

25 December 2020 2,452 1 View

Health or economy !?

With the continuing threat of the Coronavirus and the deterioration of global economies due to quarantine, are we heading towards choosing between health or the economy?

29 April 2020 4,509 26 View

I need experimental researches that clarify the effect of well number and width on the performance of quantum-well solar cells?

Dear All, I need experimental researches that explain the tradeoff between short circuit current and open circuit voltage when we increase the number of QW, or width. Thanks in advance

30 April 2019 2,941 2 View

What is the most suitable PEG to use to link peptide in a peptide-coated niosome?

We are working on preparing a drug carrier niosome. We are planning to use a 24 amino acid peptide (MW=3000 kDa) as a coating which will serve for homing purposes. As to link the peptide we are...

31 October 2018 5,295 1 View

Why is isolated liver mitochondria and colon mitochondria respond differently towards the same agent?

In a recent experimentation where we tested an agent's effect on the oxidative stress status on mice (inflammatory model of IBD), we found that livers and colons present a similar response...

24 September 2018 9,990 1 View

How to determine R-cuve for concrete?

Dear all, How to determine R-curve of concrete for crack growth, is there any equations to calculate it ? If it is related to experimental test, is there any data of r-curve in order to...

15 November 2017 4,314 1 View

Human T lymphocyte activation?

hey...I m planing to activate human T cells after asolated from PBMCs and before, so I know some about PHA that can activate T cells. also the compaine between PMA and Ionomycin also can activate...

30 August 2016 6,913 3 View

Parameter of Archived multi-objective simulated annealing (AMOSA)?

01 May 2016 7,756 4 View

How can I define the dimensions in multi dimension scaling (mds) to build a perception map?

dear researchers, i need to develop a perception map through a multidimention scalin (mds) on spss in the framework of measuring brands image, but i need to know how can i define the dimentions....

06 December 2015 6,784 2 View

Can I simulate crack propagation on ABAQUS ?

Good morning every one. I need your help. I am now working on crack propagation on concrete gravity dams, I want to know some models to simulate penetration of water on cracks and how it...

17 November 2015 3,116 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View