How is geometric mean a better average than arithmetic mean, for Cq of the reference genes?
Devi Prasad - Thanks for pointing that out. I think the arithmetic average is better.
True. In your .doc file, I feel it should be AM. In Pfaffl's formula (paper link attached), if we substitute GM of [Eref1^(delta Cp ref1)], [Eref2^(delta Cp ref2)], [Eref3^(delta Cp ref3)] in place of [Eref^(delta Cp ref)], then we arrive at your formula, with an AM. In short, GMs of (E^Cp) translates to AMs of (Cp*log2 E)
http://nar.oxfordjournals.org/content/29/9/e45.long
Correct - am familiar with that paper too. Just looked into the mirror of numbers backwards is all. Caught the mirage instead of the fish. Thanks.
The attached file now incorporates this adjustment. Thank you Devi Prasad V.
Another file confirming this from Dr. Amy Replogle of ResearchGate (attached).
Thank you Amy~!
The attached .xls file from Dr. Replogle is very useful. Is it possible to obtain the .doc file that is mentioned in comments of that file? Most comments refer to specific equations in a .doc file. Thanks!
Dear Atticus, sorry late reply on this. Perhaps you can ask Amy directly at:
https://www.researchgate.net/profile/Amy_Replogle
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