How to analytically describe a band pass filter Gaussian pulse?

I measure pulses on some background and my measurment system has an analog band-pass filter. I usually still have to do extra band-pass filtering digitally afterwards in order to remove the...

29 January 2020 3,494 4 View

Does anybody have an Android ".csv" Malware Data-set?

17 December 2019 761 3 View

What is a good RNA extraction method for RNA-Seq (especially QuantSeq)?

Which RNA extraction method would you recommend for RNA-Seq of snap-frozen fetal murine tissues? We will be doing Lexogen QuantSeq. Considering Qiagen RNeasy (good, but pretty expensive) or Thermo...

02 October 2019 9,527 3 View

Where can I find sizes of fetal mouse organs?

Does anyone know a resource for data on organ sizes (mass, preferably also dimensions) for fetal mice at various stages of development?

14 March 2019 2,229 1 View

Extracting RNA from FFPE tissue using Covaris/Bioruptor sonicator?

RNA extraction from laser microdissected FFPE tissues is markedly enhanced by sonication (paper below). However, I could only find a kit and protocol for Covaris sonicators. We only have a...

20 December 2018 2,932 1 View

How to extract bacterial DNA from swabs using bead beating?

We are happily extracting bacterial DNA using a bead beating protocol. Now we need to start processing swab samples containing fecal etc bacteria. If we put the entire swab into the bead beating,...

26 June 2016 333 4 View

Purifying a linearized plasmid?

I'm having trouble with contamination of linearized plasmid with the intact circular plasmid. I have heavily overdigested the plasmid, gel purified, and digested with another restriction enzyme,...

25 November 2013 1,907 5 View

Stability of sodium butyrate?

I'm using sodium butyrate in cell culture, but I'm slightly worried about the stability of my reagents. There is very conflicting information available regarding its stability in aqueous or...

10 March 2013 6,549 2 View

Tissue processing for laser microdissection and RNA isolation?

Hello! May I ask for your recommendations for tissue processing methods for laser microdissection and subsequent RNA isolation? I can think of at least the following protocols, each with...

12 February 2013 1,553 15 View

Low signal intensity in RT-qPCR (Cy3 probe, MX3005P)

I have problems getting very weak fluorescence signal from my probe-based RT-qPCR assay. The Ct is not bad (less than 30, and I see a clear although weak band on gel), but the signal intensity is...

17 January 2013 1,760 3 View

Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

02 March 2021 7,220 2 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

Statistics: include groups of respondents made by one person for analysis?

Good morning, I could not find literature about this so I am asking. I am organizing data for a questionnaire (N=100) that foresees some personal questions like level of education. For the aim...

25 February 2021 2,090 4 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Which statistical test to use for comparison of different groups in a categorization?

I am doing a study on how programmers with different levels of expertise choose class names. A description was provided to the participants together with a list of possible class names for the...

21 February 2021 3,662 4 View

Statistical Tests for Quantitative Study?

Hello Everyone, I have one population, sample size 200. and needed to find the correlation between Variable A and B. Also, which T-test should I choose for hypothesis testing Please advise...

21 February 2021 7,591 4 View