Predicting Depression Levels Using Social Media Posts

The use of Social Network Sites (SNS) is increasing nowadays especially by the younger generations. The availability of SNS allows users to express their interests, feelings and share daily...

01 January 1970 4,400 2 View

What is the role of asparagine in cancer?

Asparaginase is useful in the treatment of leukemia as it degrades asparagine. What role does asparagine play in the proliferation of cancer cells?

01 January 1970 4,720 3 View

Impact of the Covid-19 pandemic on the Aquaculture and Fisheries sector.

Hello all, With all the lockdown regulations, sales and trade may not be as it was before, goods and services are impacted; fish and fish products and services are not left out. Would you...

01 January 1970 3,700 13 View

The Role of Renin-Angiotensin-Aldosterone System in the Severity of COVID-19 in Diabetic/Hypertensive Patients

Hypertension, diabetes, and cardiovascular disease are the most prevalent comorbidities in patients with COVID-19. Although they do not appear to affect the infectivity of the virus, they do...

01 January 1970 8,859 8 View

The 'Biochemistry' of permed hair

Is there more to a permed hair than the alteration of the disulphide bonds between adjacent strands of protein in hair?

01 January 1970 8,589 3 View

'Biotechnology'- A textbook authored by V. Satyanarayana

Please who can help with copy of this textbook in PDF?

01 January 1970 5,980 5 View

Cancer cells and metastatic phenotype

Why do cancer cells use distinct transcriptomic and proteomic programs to reach the same metastatic phenotype?

01 January 1970 4,948 3 View

Biotechnology: Do the Benefits Outweigh the Downsides?

The major apprehension of genetic engineering is that through recombinant DNA experiments , unique micro-organisms or viruses (either inadvertently, or sometimes deliberately for the purpose of...

01 January 1970 965 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

What are the possible effects of COVID -19 Vaccine/s on the persons already recovered from the disease?

Persons recovered from COVID -19 are having antibodies against the virus inside their bodies. They are having T cells already sensitized to the antigens of the virus. If they are vaccinated (may...

12 February 2021 4,106 22 View

What is the reason, we got both test and control band on LFA in negative sample?

I am working on Lateral flow assay for virus detection by carbon conjugated polyclonal antibody. We got a high intense band in both test and control line on LFA in negative sample used as buffer....

11 February 2021 6,558 2 View