What is the optimal approach: preparing the differentiation medium (including DMSO) and storing it at 4 degrees Celsius, or preparing smaller volumes just before use?
Prior various seismic events stress accumulation tajkes place .Its symptomatic siganals are displayed in the tectin region by subsurface condison varaion as in Geology Geomorphology ,Thermal...
01 January 2024 6,361 5 View
I have Force-Displacement values of a tensile test that undergoes uniaxial loading. Please find attached the stress strain curve of the loading. Sigma1 denotes the Equivalent stress of the...
06 December 2023 8,675 1 View
Any thoughts on this?
23 October 2023 8,131 0 View
1) Exploratory Case Study on Performance of TCP Congestion Control Algorithms, MAC Protocols, and Routing Protocols for Telemedicine in Wireless Network Environments 2) Comparison of the...
05 May 2023 5,346 4 View
I I isolated Soybean DNA from leaf samples with CTAB method 6 times but each time agarose gel does not shown the band's I already reprepared the stocks 3-4 times
17 March 2023 7,027 3 View
I want to compare CI between three diseases in single curve. I am also interested to know the code for decomposition analysis of alcohol behavior in STATA.
17 March 2023 565 0 View
What is the appropriate method of including the heat generation in numerical modeling of friction stir welding process due to threaded profile of cylindrical pin .
16 October 2022 1,402 0 View
I am looking for tutorial/Code on Latent Class Analysis using R where i am able to compute Entropy, BLRT and VLMR using R . I used Jamovi software to identify AIC, BIC, Goodness of Fit and...
30 July 2022 357 1 View
How to compute Entropy, BLRT and VLMR for Latent Class Analysis using R software? I am looking for tutorial/Code on Latent Class Analysis using R where i am able to compute Entropy, BLRT and...
30 July 2022 4,145 0 View
I am looking for new clinical studies using enhance brain waves to improve memory impairments and learning capabilities?
26 May 2022 4,618 2 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...
19 July 2024 8,522 0 View
I am conducting free radical polymerization using Kraft lignin and MMA, with DMSO serving as the solvent and there is also an initiator. Following the reaction, I obtain a solution, but I am...
15 July 2024 9,827 1 View
I'm using Dorsomorphin (C24H25N5O) as one component in dual SMAD inhibition of pluripotent stem cells to differentiate into neurons alongwith SB431542. Now as to solubilizing 10mg SB in DMSO for...
11 July 2024 4,832 0 View
I have a very polar alcohol (soluble in MeOH, H2O, partially in heated DMSO). And a long chain fatty acid that I would like to couple. I can't find a common solvent for them. I can form the...
24 June 2024 9,319 0 View
I saw the iPSC about a week after holding the stock (12 well and 3 stocks) and there were very few cells attached, and some cells were differentiated. I wonder what the problem is. The plate is...
12 June 2024 6,087 0 View
I have seeded HeLa cells in a calculation of 10000 cells per well in a 96 well plate. Then I went for treatment in the next day. Then at last, I added 7ul of 5mg/ml solution of MTT reagent into...
12 June 2024 1,460 3 View
Is it possible to do FACS on snap frozen cells ? They were isolated and directly dropped in liquid N2. They were not frozen with DMSO or FBS.
11 June 2024 8,915 0 View
Hello, I discovered that a compound that I use, which get integrated into RNA, might have an unspecific impact on mRNA stability of a gene of interest. To confirm that, I pretreated my cells for...
10 June 2024 276 2 View