Dear,
I am trying to use a 1% agarose gel to replace the current in use HistoGel from Richard-Allan scientific. The HistoGel has tend to dissolve when it goes thru the tissue processor (excelsior).
Now my 1% agarose has dissolved as well. Only some some fragments of the lager pieces where found back.
Please help?
Paul Rutland
are you checking the temperatures in the processor reagents. It sounds like they are too hot and agaroses will redissolve when over heated. Perhaps you have a problem with a thermostat regulator on the processor
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Laura Verheijen
It was a heating problem. A new tissue processor has fixed the problem and the HistoGel is stable. Thank you
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