You can go ahead and add sterilized nutrient broth to your culture, and under sterilized conditions try to scratch the fungus growth. After that shake the tube overnight at 130-150 rpm. It will be active after that
Hello Fatima to avoid bacteria use both tetracycline 2.5 ppm / 1 L , Penicillin 5 ppm / 1 L. Use Dichloan ( botran ) fungicide to avoid non septation fungi ( Rhizopus spp) .Use Benlate to avoid the septat fungi