dilute it in ethanol or dmso?
which solution is better as i used 95% of ethanol as my extraction solvent?
It depends on the rest of your work. If it is for analyzes, you can resume your extract with ethanol or a solvent appropriate to the nature of the analyzes you are going to do. On the other hand, if it is for the study of biological activities, you eliminate toxic solvents (alcohols or others) and you use water. If you have a solubility problem, the use of DMSO becomes necessary.
If you know something about the compound that needs extraction, one can choose a solvent.
Methanol and ethanol are commonly used since they extract a wide range of compounds.
Another set of solvents is hexane (or petroleum ether), followed by dichloromethane, ethyl acetate, methanol (or ethanol), and finally water. Depending on the compound(s) that show biological activity, it can be in more than one of the extracts depending on its solubility in the different solvents.
Hello, first of all, what is your analytes or general analyzes?
Second, Complementing the comments above, sometimes a mixture of solvents is needed to extract all target-components. I recommend the use of mixture design to evaluate the best/optimal mixture condition. This book of Dr. Cornell is a great start point for the use of this chemometric tool:
https://books.google.com.br/books/about/Experiments_with_Mixtures.html?id=piWpe3yEAjYC&source=kp_cover&redir_esc=y
Hi
It is necessary to know about the of the aim of the dilution to choose the best solvent.
For example if you are going to analyse a specific type of compound regarding to the selected method (such as HPLC, GC or MS analysis and ...) and solubility of the target compound(s), you should chose the solvent which be suitable. If you are going to analyse in GC-FID or GC-MS, you will need the solvent with low evaporation point. If you want to use reversed phased HPLC, its better to use water or the common solvents used in HPLC like methanol or acetonitryl.
On the other hand if you are going to use the diluted solution into a reaction you should consider the best condition for the reaction.
In general we use ethanol because it extract the wide range of components, for example for extraction of essential oils from mint plant we use ethanol, for analysis the sample by instrumental analysis we dilute it by suitable solvents like methanol or acetonitril. However you must know every interferences in your extraction methodology for choosing suitable solvent.
For diluting the yield, you have to know the nature of the compound ( polar or non polar). Then it is very essential to know the stable temperature of the compound.
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