Hello! Please, how does fold induction differ from fold change? and which of the terms is best used for qPCR result interpretation?

05 May 2018 1 2K Report

I`m working on gene expression study and sometimes the two terms confused me.

I do not know. I think the folding problem, in general, at least for DNA, RNAs, is misconstrued... The deeper problem is a different kind of coding...

Badges
Science topic

More Ibrahim Bala Salisu's questions See All

My question concerns MTT cell viability assay?

Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...

21 July 2024 9,914 1 View

Guidance needed for preparing the hydrogel samples for the XRD instrument?

I'm working with a hydrogel sample and I'd like to perform XRD analysis. Can anyone offer guidance on preparing the hydrogel for the XRD instrument? Specifically, I'm unsure about the best method...

20 July 2024 3,611 4 View

How can I extract the mathematical equation from existing Neural Network Model?

There exists a neural network model designed to predict a specific output, detailed in a published article. The model comprises 14 inputs, each normalized with minimum and maximum parameters...

14 July 2024 2,714 3 View

Why does my IVT gRNA product gives HMW smear in gel?

We are using MEGAshortscript™ T7 Transcription Kit for transcribing gRNAs from linearized plasmid templates. (MSP3485 digested with HindIII) (https://www.addgene.org/140082/) We let the reaction...

01 July 2024 6,342 0 View

How can Abaqus CAE be utilized to assess the variation in wall thickness throughout a plastic bottle following a blow molding simulation?

Hello ResearchGate Community, How can Abaqus CAE be utilized to assess the variation in wall thickness throughout a plastic bottle following a blow molding simulation?

26 June 2024 9,281 4 View

JCR 2024 Impact Factor Download File-Factors caused decline in IF are?

Why the IF of almost all journals declined so much? For the file download go to https://www.researchgate.net/post/JCR_Impact_Factor_2024-Factors_causes_decline_in_IF

21 June 2024 3,358 0 View

When use a HOMER Pro to design hybrid system PV/electrolyzer/hydrogen tank. How can I use all electrical load to power the electrolyzer?

Simulating a Hybrid System with PV, Grid, Electrolyzer (No Load) in HOMER. here is a question about how to use HOMER Pro to design a solar PV system for water electrolysis using electrolyzer for...

05 June 2024 5,609 0 View

Expert questionnaire, Is it possible for people with a specialty in music psychology to evaluate this questionnaire? Through the link below?

It is possible for people specialized in music psychology to evaluate this questionnaire Through the link below Thank you https://forms.gle/qzoD7jN2UDUhMjwQ7

02 June 2024 9,546 0 View

My transduced cell can resist Puromycin and 99% express GFP, BUT can not show me the difference in the knockdown. what could be the possible reason?

My transduced cell can resist Puromycin and 99% express GFP, but it cannot show me the difference in the knockdown. What could be the possible reason?

31 May 2024 454 2 View

How can we extract DNA from yeast isolates without using any lysis buffer or lysis enzymes? Is there any manual protocol?

31 May 2024 548 4 View

Using OBD technique i am trying to measure laser induced shockwaves velocity i found that at start velocity increases and then decay?

i am unable to interpret why its increases in start as shown in figure

11 August 2024 2,179 1 View

How can I interpret the data without the need of solving it manually?

How can I interpret the data gathered without solving?

03 August 2024 9,054 3 View

How to prediction 2D to 3D aptamer?

Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...

30 July 2024 6,794 0 View

Why only alpha wave of brain are found in some patients?

I have found an EEG where only alpha waves are present. Beta waves are not found in active patients. What interpretations ?

26 July 2024 4,741 1 View

How to interpret results of AST for Pseudomonas aeruginosa?

While doing AST for Pseudomonas aeruginosa, after incubation, no zone of inhibition observed in the plate near the well. wells surrounded by bacterial growth, when the same plate observed under UV...

25 July 2024 9,229 1 View

Does anyone know the scoring and interpretation for Psychological Birth Order Inventory (PBOI) of White Campbell?

Hi, I am currently a upcoming 4th year student who is need of your help as I couldn't find any accessible file for the manual scoring and interpretation for the PBOI - White Campbell. My group and...

23 July 2024 2,269 1 View

Can you please interprete this analysis?

This is a analysis on IHC scoring of WNVs and USUV performed with culex pipiens

22 July 2024 1,880 0 View

Correct statistical approach for a hermit crab shell exchange experiment?

Hello, I'm planning a shell exchanging experiment with two marine, hermit crab species inside of a tank. I only have one tank available for this experiment. I plan on running 30-40 shell...

20 July 2024 5,676 3 View

How to interpret a Low R squared and negativ adj. R on my fixed effects panel analysis?

Hi guys, in the context of my master thesis i analyze the statistical relationship between income and subjective well-being (Panel: SOEP, n: 300.000 observations over 10 years). After creating a...

13 July 2024 7,539 6 View

What is an ideal threshold value for log2(fold change)?

Hello everyone, I am performing differential gene expression analysis and I am not sure what is an ideal threshold for log2(Fold change).Thanks in advance!

09 July 2024 1,437 1 View