Dear Dr. Amjad Khan

I have not tried, but I can share an article that will be helpful.

Most stripping methods tend to affect tissue integrity or slide adhesion. There is a possibility that the stripping technique may alter the target confirmation resulting in decreased signal intensity. Nevertheless, I have attached a paper below in which the investigators have evaluated several antibody stripping techniques. They have come up with a technique that would result in a complete, efficient, and reliable stripping of a broad array of antibodies while preserving tissue adhesion on the slide and maintain similar levels of antigenicity in FFPE samples, across multiple rounds of stripping and staining.

They have found a protocol using β-mercaptoethanol and SDS to be most reliable for stripping antibodies across multiple rounds of staining and minimizing the odds of cross-reactivity, while preserving tissue adhesion and antigen integrity in tissue. Make sure that you carry out proper validation and optimization of antibody stripping steps.

Please refer to the article.

https://pmc.ncbi.nlm.nih.gov/articles/PMC7269157/

Regards,

Malcolm Nobre

Dear Dr Malcolm Nobre thank you for sharing the paper.