Dear Taha,
The best way is to pick study cohort from hyper endemic areas . It is well documented that mostly a booster dose is often required after 6 or 7 years of vaccination. The Serum Anti-HBs titre is measured , if
To assess the immunologic profile of individuals who have been vaccinated against Hepatitis B Virus (HBV) for 10 years, you can conduct a comprehensive study that evaluates the persistence of protective antibodies and other immune markers. Here are some steps and suggestions for your research:
- Design a longitudinal study that follows individuals who received the HBV vaccine 10 years ago.
- Consider a cross-sectional approach where you collect blood samples from a representative sample of individuals who received the vaccine.
- Obtain informed consent from participants.
- Collect blood samples from the participants.
- Measure the levels of HBsAg-specific antibodies (anti-HBs) to assess the persistence of protective immunity.
- Assess other relevant immune markers such as cytokines, T-cell responses, and immune cell populations to gain a comprehensive immunologic profile.
- Analyze the data to determine the percentage of individuals who still have protective levels of anti-HBs antibodies.
- Examine any changes in T-cell responses or other immune markers.
- Compare the immunologic profile of these individuals with those who have not been vaccinated against HBV.
- Compare with individuals who were recently vaccinated to see if there are any differences in immune responses.
- Use appropriate statistical tests to evaluate differences between groups.
- Perform correlation analysis to identify any relationships between immune markers.
- Ensure that the study is conducted in accordance with ethical guidelines for human research.
- Protect participants' privacy and confidentiality.
- Summarize the results and conclusions.
- Discuss the implications of the findings for long-term immunity and potential booster recommendations.
- Consider publishing your findings in a scientific journal to contribute to the body of knowledge regarding HBV vaccination and long-term immunity.
- Collaborate with experts in immunology and infectious diseases to ensure that your study design, data collection, and analysis are scientifically sound.
All the best
You will need to:
1. Study design should be cross-sectional.
2. Obtain ethical approval. Once granted, have a consent form ready.
3. Sample type should be blood, from which serum will be obtained. But this depends on the scope of your research.
4. Analysis can be done using ELISA.
5. Since you intend to look for the persistence of vaccine-induced antibodies (anti-HBs) 10 years post vaccination, a quantitative analysis is mandatory. Data generated using the qualitative antiHBs ELIZA kit will be able to provide quantitative data as well using MS excel.
6. Subject data to statistical and descriptive analysis.
Puede ser a través del uso solo ADN de sangre en lugar de muestras de células frescas.
Existen millones de muestras de sangre almacenadas en biobancos de todo el mundo, muestras que ya existen por otros motivos.
The fist thing to know the vaccine strain used dose ,titer ,effect of booster dose,then study immunological status of vaccinated person before vaccind and after vaccination,need for booster dose or not the select your aim
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