DC on my compound treatment is secreting high amount of NO, and it is well known that it leads to the nitrosylation of TCR on T cells. So, is there any in vitro method to check it.
We have used the Griess test. Check out ( Free Radic Biol Med. 2007 Sep 1; 43(5): 645–657).
Dear Sanpreet,
please check out the latest publication from my PhD lab, which includes in vitro assays (antigen presentation and T cell activation) as well as pre-clinical models of autoimmunity.
https://www.liebertpub.com/doi/full/10.1089/ars.2019.7788?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmed
All the best & kind regards,
Michael
Thank you all, but griess reagent tells only about the NO level. But how we can check weather high NO released by DC are inhibiting the T cells through TCR?
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