Haneyeh Shahbazian I think 3k is ok and up to 10 k is also fine but I wonder what the starting conc. of your drug for loading and conc. of liposome. Also, I think the speed is too high if your liposome is not stable in RT. So, I recommend using 7000 g for 10-15 min with. proper temperature.

Hi,

You mentioned two numbers for MW of your drug (455 or 456?).

If it is 456 g/mol, there are several compounds with these specifications:

MF: C22H22ClN5O2S

MW: 456g/mol

and they vary in their Mean Hydrodynamic Particle Size (required to determine molecular cut off of your filter). Also what is its charge?

Therefore, your question can only be precisely answered if we know particle characterisations of your drug and physical stability, Zeta potential, PDI and size of your liposome formulation.

I agree with @Achuthamangalam Madhankumar normaly molucular weight wont affect and you can do 2000g at the range between 30-60 as suggest

How to insert DOTAP into a liposome drug (lipo-dox *doxorubicin) to enhance encapsulation for hydrophobic compounds?

1. Here I'm working on liposome drugs modifications, which I use Lipodox (HSPC: Chol: PEG2000-DSPE= 3: 2: 0.3), when I tried for the first time, there was a white transparent membrane in the flask bottom after evaporation using HEPES 10 mm 80 microml, so far I read many references it should be clear membrane. The condition is 50 degrees C; 1 hour before adding HEPES).

2. Here also using DSPE-PEG-Maleimide (3.4 DA), which will be attached to the liposomal surface, which percentage is proper for this modification, since (point 1, has a white membrane on the flask), is this effect to the DSPE-PEG-Maleimide attachment?

3. Is there any suggestions, which better way to combine DOTAP into a liposome drug to enhance the encapsulation itself? Since using Lipodox and which best ratio condition for this?