I have tried heat, heat +EDTA and a reextraction but they did not work, at least with plant tissues.
Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...
15 July 2024 4,615 1 View
Occasionally we observe lack of staining in the inner part of a skin section processed with indirect immunofluorescence. Can be due to incomplete removal of the cryoprotectan during the wash? Any...
07 July 2024 8,200 2 View
In most of the literature I am reading at the moment, the abbreviation μg m−2 h−1 is used to express mean annual fluxes of greenhouse gases. I am confused by the "h" and supposed it stands for...
27 June 2024 9,633 1 View
Do you think the use of virtual reality with dyslexia students is good?
23 June 2024 8,437 2 View
I'm analysing a novel in which the said emotion is fundamental to understand the development of characters. Thank you in advance.
22 June 2024 4,859 8 View
I am doing a thematic analysis from interviewees done with my main group of interviewees. I also have done two interviews with another group of interviewees which provided interesting insights...
21 June 2024 3,305 6 View
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19 June 2024 2,125 3 View
Hi, I am doing western blotting of isolated extracellular vesicles, and I have albumin and calnexin contamination that I don't know why. Is there any way to avoid it?
16 June 2024 9,907 4 View
I am going into my inductive thematic analysis with three research questions. In the results section, is it okay to structure it according to these three research questions, presenting the themes...
16 June 2024 1,116 3 View
Please note, that this is my first manuscript under the name Mária Čápová. Then I have been married, and my name change to Plachá, but I would like to have this article in my profile. Ontogenetic...
16 June 2024 7,178 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it
06 August 2024 947 1 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?
04 August 2024 1,537 1 View