Hello everyone. Please tell me that what amount of estrogen and estradiol should be used for MTT and immunoblotting assay?

10 October 2018 0 3K Report

Estrogen and estradiol, MTT and immunoblotting assay

Badges
Science method

What about the topical application of estrogen on wound healing ?

The application of estrogen to wounds reduced inflammatory responses and promoted angiogenesis and woundcontraction more than the two other standard treatment methods.

09 July 2024 8,236 0 View

Hi all, I used water as my negative control in my immunoblot experiment, but i can see a band in this lane, could any one tell me why?

Hi all, I used water as my negative control in my immunoblot experiment, but i can see a band in this lane, could any one tell me why.

06 May 2024 7,222 5 View

Problem with YES estrogen bioassay. Estrogen contamination?

Hello everyone, I've been encountering a recurring problem with the YES (Yeast Estrogen Screen) test, specifically related to the development of an intense red color after adding CPRG. I'm using...

07 February 2024 1,144 0 View

What is ideal concentration of antigen for development of Test line ? Does the antigen should be with BSA/OVA?

1. If we looking for detection of estrogen in my sample. the concentration of antigen in sample should vary but the Test line should be same concentration. If I choose a BSA-bound estrogen of...

22 January 2024 3,745 1 View

Do old male mouses still have the Estrogen in their body?

Hello I am working on a research topic related to Estrogen function in Acetaminophen-induced liver injury on male mouse model. We only have some old male mouses. I wonder if these old male...

17 October 2023 9,034 0 View

Is there any induction for Estrogenic activation?

I have tried many times to examine estrogenic activation for some compounds , via Luciferase assay the results indicate the compound is estrogenic and sometimes is not, what do you think the reason ?

30 July 2023 747 1 View

Western Blot trans-blot turbo transfer system of 1.5mm PAA 15%?

Hello, I have to transfer a PAA 15% of 1.5mm onto a PVDF membrane in the Biorad Trans-blot turbo transfer system. The protocol says for a mini gel to use 1.3A up to 25V for 10 min. I read about...

12 June 2023 9,685 1 View

Levels of estradiol in intact females odderly high?

Dear all, I am measuring the levels of estradiol in plasma in intact B6 females around 18 weeks old, using QuickStep Mouse Estradiol ELISA competitive assay (QSES033). I am getting for all mice...

21 April 2023 3,371 0 View

Does MCF7 tumorigenesis in female nude mice require exogenous estrogen?

If estrogen is not exogenously given, can MCF7 cells grow into tumors under the skin of female nude mice?

06 February 2023 9,986 2 View

Is cloudy urine possibly an effect of estradiol pellets or fulvestrant-resistant cells in mice?

I have established tumors in mice using fulvestrant-resistant breast cancer cells. These mice also have estradiol pellets inserted under the skin. Some of the mice have thicker/cloudier urine that...

11 January 2023 9,991 4 View