Estrogen and estradiol, MTT and immunoblotting assay
Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...
22 February 2021 4,352 2 View
So I'm new to lab research and learning Western Blots these days. I ordered my secondary antibody and it arrived in a powdered form. Here is the...
27 January 2021 5,402 3 View
Sometimes my western blots have very uniform/even background, other times they're very uneven and patchy like in the attached image. Why is this? I thought my blots were drying out during...
05 December 2020 8,656 5 View
In the past, I've always used Licor's old blocking buffer that included sodium azide. I would dilute my antibodies in the buffer, stain, re-collect the dilution, and re-use it a few times. The...
28 November 2020 5,795 3 View
For western blots, I normally stain with 10 mL buffer with 1:1,000 dilution of my specific antibody. Recently, there is an equipment change in my lab and 10 mL isn't enough volume to cover the...
27 October 2020 2,749 7 View
A few years back I tried several available Hif1a antibodies on frozen sections of heart and lung. None of them worked very well. Most worked well for immunoblots. Have there been any advances in...
12 October 2020 7,727 2 View
Dear Colleagues, Can anyone tell me if there are Data about hormone levels in male mice , E2 Estrogen, and Testosterone blood levels in male mice. Thank you very much.
20 September 2020 1,035 3 View
Dear fellow researchers: I've recently started performing WB, mostly with molecular weight markers to test the equipment and optimize I/V conditions. This time I also ran protein lysates from two...
12 August 2020 3,943 11 View
When using abcam anti-alpha-tubilin on immunoblotting developed on ECL to probe tubulin on mice splenocytes, I see a clear band at 55kDa but there appears consistently another band at about 35kDa,...
26 July 2020 2,158 4 View
As per available records, PCOD develops due to imbalance of the two female hormones (estrogen and progesterone) where estrogen taking the upper hand. Common symptoms are: 1. Menstrual...
01 February 2020 2,104 10 View
