Do I have to use probes for real time pcr? I couldnt get the idea of probes, I think. Thank you:)
Not necessarily.........
Dear Beyza you can quantify your target using SYBR green chemistry. Which does not need a dye labelled probe. This chemistry needs only sense and antisense primers. you can purchase the SYBR green form Applied biosystem or Sigma chemicals etc.
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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
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