Hi Moez

I would not recommend using a DNA extraction kit for this. You may extract DNA from ethanol stored parasites (adults) using 200 μL of a 5%suspension of deionised water and ChelexW (BT Chelex® 100 Resin BIO-RAD, Hercules, USA) containing 0.1 mg/ml proteinase

K, followed by incubation at 56 °C for 3 h, boiling at 90 °C for

8min and centrifugation at 14,000 rpmfor 10 min. You can use the PuRe Taq™ Ready-to-go™ PCR beads GE Healthcare (Buckinghamshire, UK) for the following PCR reactions.