How do you choose the detergent concentration for protein purification?

hey i am using 2%OG and 1%DDM what concentration do i need to use with DM? is there a formula to calculate? Why usualy using 1%DDM and 2% OG (what % is higher then the CMC?) thanks

31 December 2018 3,925 8 View

Is using synonyms or antonyms enough in paraphrasing a text? Is doing this considered Paraphrasing?

When coming to write a research paper or a report, most EFL students tend to paraphrase through using synonyms and antonyms. Thinking that it is enough. How can we deal with such a thing?

26 December 2018 5,894 15 View

How to calculate the sample size? Can I cite the same article(s) in more than one research?

I'm planning to do a research based on questionnaire/ survey and population are clinical researchers from the whole country. The count of researchers in the country in unknown (I don't know how...

07 March 2018 2,405 4 View

In survey, not all questions answered by respondents. What should I do ?

for example, total respondents are 100. 50 answered Q6, 45 answered Q7 , 60 answered Q8. What should I do to write the results to be acceptable for the journal reviewers? The survey has...

04 February 2018 1,164 4 View

When dissolving expanded polystyrene (EPS) in acetone what fumes are produced? And is there a way of measuring this?

I am researching and developing alternate methods of recycling EPS and am getting interesting results via dissolution in acetone. However I want to make an evaluation of the potentially hazardous...

15 January 2018 478 2 View

How do calculate decolorizing activity of bacteria by using uv-vis spectrophotometer?

27 June 2017 596 3 View

How can i produce genomic analysis (DNA extract) with very low concentration of DNA from Yeast?

hey all i need to extract DNA from yeast cells. but how can i do it from one yeast or very low number of yeast cells. is there a special protocol for it? Thanks May

17 June 2017 5,842 9 View

Does exist a gene expression programming toolbox for matlab?

20 May 2017 3,852 4 View

For recharging Hitrap Talon 1ml cobalt, can I use Cl2Co*6H2O?

The GE official protocol says I need 50mM Cl2Co, so should I calculate only by the Cl2Co MW? Thank you!

30 April 2017 3,961 2 View

What settings should i be using on Primer3 and Primer BLAST to prevent primers with potential off-target products from showing up in my results?

22 March 2017 6,290 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

No title

02 March 2021 3,060 3 View

No title

02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View