Hello, Does anyone have experience in recording of the spreading depolarization in mouse brain using Axopatch 200A in I Clamp mode?

Volodymyr Gerzanich @Volodymyr-Gerzanich

09 October 2023 1 6K Report

Suggestions on electrode resistance? Can one get away without Faraday cage?

Péter Gyarmati

electrode resistance for mouse neuron should be around 15-30 pF

https://support.moleculardevices.com/s/article/Axopatch-200A-selection-CV-headstage

can get away wo Faraday cage? - depends on the building/room you want to use it in, but the answer is 99% no

Badges
Science topic

Similar topics
Entomology
Resistance

More Volodymyr Gerzanich's questions See All

Journal of nuclear materials 595 July?

Contents of the issue

30 June 2024 4,088 1 View

Can anyone recommend any medical or multidisciplinary journals and websites for possible publication of scientific articles in them?

Good afternoon, dear scholars! I am creating this post so that we can communicate on this topic: Can anyone recommend any medical or multidisciplinary journals and websites for possible...

23 April 2024 7,962 0 View

Can anyone recommend any medical organisations or associations for membership and exchange of experience?

Good afternoon, dear scientists! I am creating this post so that we can communicate on the topic: Can anyone recommend any medical organisations or associations for membership and exchange of...

23 April 2024 2,542 0 View

Can you help me choose a method of treatment that is effective in severe, unbearable pain?

The patient is 4 days postpartum after a physiological delivery. Complaints of unbearable pain in the perineum and lumbar pain. There were minor internal tears. On examination, there is no...

25 October 2023 3,602 21 View

Dear Colleagues! Why do many people consider plowing a harmful operation?

In my opinion, if plowing is done CORRECTLY, then the problem of restoring soil fertility can be solved.

19 September 2023 6,355 2 View

Why doesnt my sequncing work with Forward primer ?

I am having a problem with the sequencing of recombinant plasmids. I need to get sequences in both forward and reverse directions. For sequencing, I am using M13 Forward and Reverse primers...

29 May 2021 8,846 3 View

Help me to get series already generated by RBC method: predicted and actual time series of USA about annual growth rates of GDP, Hours worked, etc?

I am working on an advanced version of the Real Business Cycle theory, RBC, and I want to compare my results with the ones of traditional RBC. So that I need the following time series already...

15 March 2021 3,892 2 View

Dear colleagues! What are the characteristics of the essence of animation?

On the one hand, the concept of "animation" emphasizes the spiritual aspect of the relationship among the subjects of the pedagogical process, and on the other - involves active cognitive activity...

23 December 2020 6,730 11 View

Coupling of Brillian Violet 421 to antibody for flow cytometry. Could anyone share the knowledge of coupling of Ab to BV421 - protocols, kits, etc.?

BV421 seems to be very good fluorophore to use with UV laser – bright and no spills, but not too many antibody conjugates available. In my experimental set up I cannot use BV421 on a secondary Ab...

30 July 2018 5,480 2 View

Synthesis of 6-chlorophenanthridine?

We have plans to prepare some dyes and electrode modifiers, possessing phenanthridine moieties. How can we get the 6-chlorophenanthridine (in European numeration, 9-chlorophenanthridine)?

24 April 2018 5,822 1 View

What is the reason for current dropping in OER , LSV curve?

I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...

10 August 2024 3,628 1 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View

Binder for ZnO nanoparticles in graphite paste electrode?

I have been using paraffin, but the deposited ZnO still detach from electrode. What is the best binder to modify graphite paste electrode with ZnO nanoparticles?

03 August 2024 4,623 3 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,121 4 View

How to find a reaction is reversible or irreversible in CV? How to know the number of electrons transfer?

My screen printed electrode CV is given here. For Nerstean one electron reaction the delta E value should be close to 57 mV and ration of Ic/Ia should be close to 1. I did CV of my screen printed...

30 July 2024 4,671 3 View