How is linear form of plasmid circularized?

Hi all, I'm working on transforming E. coli with plasmid in a mixture of linear and circular form. What happened to the linear form once uptake? Do cells ligate them directly? Or cell DNase...

13 January 2021 8,336 4 View

How much the nanoparticles reach the inflammatory tissue?

Dear colleagues, I want to know the average analysis data of EPR effect on inflammatory tissue (acute lung inflammation/injury, ischemia/reperfusion, rheumatoid arthritis, and sepsis..) In...

22 December 2020 3,147 2 View

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02 December 2020 7,293 2 View

Why DMEM color is changed?

Why the pink color will change to yellow, when the DMEM media is frozen? however after dissolution in room temperature, media color will change back into pink.

26 October 2020 708 2 View

Is rotary evaporator a must before going through TPC, TFC and DPPH assay?

I am doing an optimization of ginger extract using doe software, firstly I extract the ground ginger with 20ml solvents of 60%, 70% and 80% ethanol using the ultrasound water bath in different...

23 October 2020 3,268 10 View

How to measure the bonding strength of transfered graphene on PDMS substrate?

Chemical vapor deposition (CVD) was used to grow graphene on copper (Cu ) substrate, then the single layer graphene was transfered onto a PDMS substrate. I want to measure the bonding strength of...

17 October 2020 3,567 3 View

What is the physical meaning of the cross term of the index performance of LQR ?

Dear All : I have a curious question. Among the index performance of the LQR, X' Q X and u' R u have the physical meaning. Now, my question what is the physical meaning of the cross term X' S...

02 August 2020 1,780 1 View

How to define the particle agglomeration in composite materials or foundry alloys and measure its size distribution quantitatively?

28 June 2020 7,614 3 View

Can LentiCrisprV2 is used to knockout mouse gene?

Has anyone tried using addgene LentiCrisprV2 (Plasmid # 52961) to knockout a mouse gene? The cells were resistant to puro after infection, however, none of my gRNAs can knockout the gene.

19 May 2020 1,180 3 View

How to design concrete with different fly ash/binder ratios but the same strength level for cost comparison?

I want to design some concrete mixes with different fly ash/binder ratios (can be as high as 80%) but the same strength level. The water/binder ratios (together with superplasticizer dosages) have...

12 May 2020 8,551 6 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Image Processing and classification using keras/Tensorflow?

Do you have some recommendations approaches on Image processing and classification of plant diseases using TensorFlow/Keras?

02 March 2021 1,493 4 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How could I process data in RCT to include it in meta-analysis?

I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...

25 February 2021 7,603 3 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

Can anyone please help me in processing SAR SLC data to generate interferogram using SNAP?

I have two scenes of the study area, one top and the other bottom. As per the tutorials and papers published, the step include coregistering the master sand slave images. However, when i try to...

24 February 2021 6,372 1 View