Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What could be the possible reason for the drastic variation in Qmax value for same adsorbent onto two different cationic dyes itself?

The adsorption capacity of the rice husk biochar was varying drastically with two cationic dyes namely Basic Blue 41 and Basic Red 09? What could be the possible reason behind this?

30 January 2021 1,420 3 View

Where to add metamaterial unit cells in microstrip patch antenna to enhance the antenna parameters?

In order to enhance the antenna parameters, meta-material unit cells are added but where to load them is more advantageous either at ground plane or at the patch side, also is it necessary to add...

24 January 2021 2,999 4 View

How to represent Input data as multinomial distribution of feature vectors?

Hello everyone, Is there any approach to generate a fixed no. of feature vectors from the sequential data(similar to time series data...) and also express the same sequential data in terms of the...

29 November 2020 5,508 3 View

Which is the best mitochondrial genome annotation tool? Can anyone provide the list?

I have sequenced the mitochondrial genome of some moths species. I want to annotate and the sequence will submit in the NCBI.

19 November 2020 8,849 1 View

How to solve the HAZE problem in Low emissivity coatings ?

Dear friends, I am facing a problem with an issue of milky Haze in the coatings developed for aesthetic applications. All the layers were deposited by magnetron sputtering having thickness in...

08 November 2020 2,853 3 View

Lithium ion cell holder for Samsung 21700 - 50E cells?

Hello everyone, I have 5 Samsung 21700 - 50 E (NCM chemistry) cells. I want to do basic charge and discharge by connecting it in series. Does anyone know if there is a cell holder available...

05 October 2020 6,133 3 View

How the surface waves are linked to the dielectric constant of the substrate of an antenna?

How the surface waves can be reduced by choosing an appropriate substrate.

04 October 2020 8,609 3 View

Which is the first complete mitochondrial genome sequenced lepidopteran Moth species?

I am preparing a research article about the complete mitogenome of moth. I want to include the details in my research article.

20 September 2020 8,151 3 View

What is the difference between PLSR and PLS-DA? Can we use PLSR for classification and prediction studies?

In all the textbooks related to chemometrics, PLS has been described as a regression technique that requires a dependent and independent variable for generating a regression line. However, in some...

17 August 2020 4,779 3 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

How to size battery banks?

I am designing a small grid connected solar PV power-plant. As the battery's capacity is specified in Ah and demand in kWh. What is the best way to size the battery bank? I have battery price in...

28 February 2021 1,638 3 View