Hello!
We have realised O2 consumption measurements using an oxygraph chamber on yeast that grew on derepressing media (they were forced to use respiration and not fermentation). Different pyrogallol concentrations (diluted in HCl) were tested and the O2 consumption was measured. CCCP was also added during the measurement, after stabilisation of the O2 consumption, to get the maximal rate of O2 consumption (Vmax), and the results were expressed as the rate of the oxygen consumption (in %) compared to the maximum oxygen consumption. One control containing a high concentration of HCl was also realised, but we obtained a weird result, where we had a higher initial O2 consumption than the maximal rate of O2 consumption (V>>>Vmax). How is it possible? And why do the yeast generally grow better in acidic environment?
Thank you!
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