I am having many samples of A, B types and extracting  subgroup of them either C or D solvent. The C-D value is my concern. Based on this aspect how to compare A, B group?

Data are like AC1, AC2, AC3.. AD1, AD2, AD3, BC1, BC2, BC3... BD1, BD2,BD3.. all measurement are independent. How to formulate A(C-D) data set? Will I take all AC-AD pair combination (e.g. 9) and so for BC-BD combination as synthetic data to compare?

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