I took the absorbance of the extract and sample with procedures as given in the OYAIZU 1986 method (attached here). Is there any formula to calculate frap in this method? I carried out the AlCl3 method for flavonoids. What formula should I use? Anyone with ideas, please help me out!
I took the absorbance with uv spec for both my sample and standard of vaying concentrations.... How should i proceed for calculations?
Conc of sample(mg/100g) = std conc*dilution factor*o.d of sample*100/std od* amt of extract taken*amt of sample extracted
Respected Sir,
Please try with the following method by Eom et al., 2007.
Eom, S.H., Cheng, W.J., Hyoung, J.P. Kim, E.H., Chung, M.I., Kim, M.J., Yu, C.Y. and Cho, D.H. 2007. Far infrared ray irradiation stimulates antioxidant activity in Vitis flexuosa Thunb. Berries. Kor. J. Med. Crop Sci. 15: 319.323.
Estimation of Flavonoids
The total flavonoids content was determined according to Eom et al. (2007). An aliquot of 0.5 ml of sample (1 mg/ml) was mixed with 0.1 ml of 10% aluminium chloride and 0.1 ml of potassium acetate (1 M). In this mixture, 4.3 ml of 80% methanol was added to make 5 ml volume. This mixture was vortexed and the absorbance was measured spectrophotometrically at 415 nm.
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