Have you had an experience with phosphorylated alpha-synuclein antibody for IHC-P which does not require antigen retrieval?

14 November 2022 0 4K Report

Necessary to find an antibody lot (phospho alpha-syn) which can be used without HIER or formic acid treatment.

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Science method

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We need effective surfactant to synthesize polymer nanoparticle. Which surfactant be more suitable?

Dear college. Which surfactants are more appropriate for the synthesis of nanopolymers containing polar group such as amine group.

12 November 2022 3,119 2 View

Dear colleagues. How can we implement the kinetics of the polymerization of aromatic amines in easier and simpler ways?

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09 February 2021 6,028 3 View

Call for Papers

#extended #cfp 21-23 September 2023 WORKSHOP: Dis/Connectivities: Anthropological Perspectives in the #SouthCaucasus University of #Bern, #Switzerland We have limited funds to provide travel...

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Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

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The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

How to convert g/kg Humic acid dose to kg/ha?

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02 August 2024 7,186 6 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

What do you consider to be the most relevant elements of EEG for studying cognitive biases?

I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...

31 July 2024 7,259 1 View

How to make a Lateral flow control line more uniform?

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31 July 2024 4,510 3 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View