How to prepare a Gaussian input file for a binary solvent?

The UV absorption energy spectrum of a molecule has been recorded experimentally using binary solvent (CH3Cl:CH3OH =10:1 v/v). I am trying to calculate the absorption energy of the same molecule...

15 March 2020 9,396 3 View

How can i track a mining area through satellite imagery (Sentinel 2 for example) ?

I'm trying to visualize the changer in a mining area over time (the new buildings, the exploitation ..) and I can't find enough documentation, I prefer to do this work in python since I'm familiar...

15 January 2020 373 7 View

Extraction of fish protein?

Simple method for extractiom of protein fish

06 October 2019 4,429 3 View

Does anyone have experience with DNA extraction from formalin fixed paraffin embedded (FFPE) tissue?

I am using QIAamp DNA FFPE Tissue Kit for DNA extraction; i followed the manufacturer protocol but i've got a smeer, what should i do. thank you for your help

05 February 2018 3,207 4 View

How we can do correction of abnormal pixels in Hyperion images using ENVI or ERDAS softwares ?

How we can do corrections of abnormal pixels in Hyperion images using ENVI or ERDAS softwares ? We do it before geometric corrections ? Before classification ? or we can do it after...

04 February 2018 7,381 2 View

How do I perform atmospheric correction in EO-1 Hyperion data? how i can remove the noisy bands and eliminate also the bad line ?

I need to remove the noisy bands of Hyperion EO1 image , i downoald the image from earthexplorer.usgs.gov as 242 seperate bands. and what is the good method in ENVI 5.3 for doing athmospheric...

28 January 2018 9,454 3 View

What is the best LPS-dose to induce chronic systemic inflammation to mice ?

Hi, I am starting a project that requires to induce a chronic systemic inflammation to mice. I try to figure out which dose of LPS I can give to mice and maybe how many times I should give them to...

21 January 2018 3,648 6 View

Resampling remote sensing image for image fusion ?

i have a hyperspectral image with 3m spatial resolution and have multispectral image with 1.6 m spatial resolution. I want to resample the hyperspectral image resolution to 6.4 m after i should...

18 January 2018 1,797 15 View

What is the effect of organic solvents on DNA conformation?

does the strucure of DNA will be conserved in organic medium?

31 October 2017 4,974 5 View

Matching between insilico PCR product size and agarose gel resultas after PCR reaction?

31 January 2017 6,987 4 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How to build fluid structure interaction simulation for elastic ring squeeze film damper in COMSOL?

How to build fluid structure interaction simulation for elastic ring squeeze film damper in COMSOL?

22 February 2021 5,408 1 View

Is there any difference between "SEP" as a non standard residue and phohophorylated serine?

I am trying to run a molecular dynamics simulation for a ligand that contains a "SEP" residue. also, I am preparing my files using Charmm-GUI using charmm36 ff, but it says that it will rename my...

31 January 2021 6,321 1 View

Can I heat my ELISA at 37C?

Hello, I am conducting a sandwich-type ELISA. It is to see if small molecules can interfere with a protein-protein interaction. As this interaction would occur in the human body, may I heat...

31 January 2021 4,271 3 View

Is it possible to do Reitveld Refinement on single crystal XRD data?

I tried to do single-crystal Rietveld Refinement on my single-crystal XRD data using the option "refinement on single-crystal data/ integrated intensity data" in Fullprof but it is giving error...

30 January 2021 7,813 3 View

Protein size measurement using Zetasizer?

The idea is to measure the hydro- protein sizes in various solvents (eg. with high ionic strength, EtOH/Water, other organic/water solvents), then, due to the extremely low solubility of some...

28 January 2021 297 3 View

Best tool to build missing residues, minimize and validate a ligand model?

I am working on a ligand that is co-crystalized to parotein, but the ligand is missing some residues that makes it appear as if it was separated into two ligands! what I need is to connect them...

23 January 2021 3,975 7 View

Can we use the single-elimination tournament method as the basis of an experiment design?

I want to conduct an experiment in the field of Human-Computer Interaction, where I test the perception of users about which image is better using different camera lenses. In the experiment, I...

18 January 2021 779 2 View

Which third party CFD Codes can be used for FSI Co-Simulation in Abaqus ?

Since Abaqus CFD is no longer available in newer versions what are other available codes for Fluid-Structure Interaction Simulation

11 January 2021 2,012 3 View