I'm buying this probe and it's very expensive. I would be grateful if you share any tips and recommendations with me so I can use it the best way as possible. My aim is to measure macrophage's phagosome acidification during phagocytosis.
Thank you!
Hi, I do not have any experience with the dye but please go through the following paper which mentions the use of the same:
http://www.ncbi.nlm.nih.gov/pubmed/?term=pathogen+cell+to+cell+variability+drives+heterigeniety
The same author of the paper above also wrote a Nature protocol paper in 2016, which contains a more detailed protocol on the pHrodo labeling
Dear Erinke:
I am Interested with pHrodo labeling of bacteria as well.
kindly could you send me the reference you mentioned before (of Nature prtocol).
Thanks.
Hi Muna,
It's this one:
Nat Protoc. 2016 Aug;11(8):1477-91. doi: 10.1038/nprot.2016.090. Epub 2016 Jul 21. A highly multiplexed and sensitive RNA-seq protocol for simultaneous analysis of host and pathogen transcriptomes. Avraham R1, Haseley N1, Fan A1, Bloom-Ackermann Z1, Livny J1,2, Hung DT1,3,4,5.
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