We perform the qf-PCR for pre-mutation Fra-X in a female with POI. The G banded karyotype showed an apparently  reciprocal translocation between the X and 1 chromosome, involving the Xq28 and 1qh. The FISH with Tel Xq showed only one fluorescent signal, and the qf-PCR for Fra-X and STRs linked to POF1 showed the  two maternal alleles and the paternal allele, but only one allele of the 4 STRs linked to X28.