Has anyone worked on spleen tissues for micro-dissection laser technique? What is the typical buffer which can protect (RNA spleen) from degradation?

11 November 2012 0 8K Report

Until now, I could not find a typical buffer, which I can use to dilute my antibodies and at the same time, keep spleen RNA from degrading? When I do the experiment, I cut the frozen spleen (- 20°C) and then I fix them by Ethanol 75%. After incubating the sample with Ab - short time- and doing the RNA extraction, I do not see RNA, even I do the experiment under ice conditions. Any suggestions?

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