We are interested in studying the expression of mRNAs in different cell types. The idea is to localize the expression of mRNAs with specific cell types in the tissue itself.
I have not used in situ PCR.
but I have been getting a fair amount of literature for a protocol called Stelaris. Basically it is a type of FISH to detect where your mRNA is in a cell.
Short version of how it works is you have a ton (60 or more) of short probes per target what each have the same fluorophore. so, each mRNA strand will bind multiple probes increasing the signal. It might be good for FFPE due to FFPE chewing up and spitting out RNA.
Thank you for your answer. Do you know someone who has used this technique, or can help me?. We could raise any type of collaboration.
I've been trying to talk my boss into trying it out. So, sorry can't help you with that. Might be able to post another question asking that though.
Why is it important for you to use in situ PCR? Is in situ hybridization not sufficient?
Dear Lang; First thanks. I'm interested in determining which cell type is responsible for the expression of my protein in the tissue itself.
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