Carly Wiersma

5 Questions 4 Answers 0 Followers

Questions related from Carly Wiersma

How can I make sure that BCTC doesn't fall out of solution/form DMSO crystals when treating cells?

I resuspended my BCTC as described on the bottle at 10mg/mL in DMSO. However, I can see the formation of crystals which I can only assume are DMSO and that the drug is precipitating out of...

15 February 2021 5,810 1 View

Why am I getting inconsistent results from my ChIP PCR?

One of the times I performed this ChIP I got strong binding at the locus I amplified, but I have not been able to reproduce this result after many many tries. However, I did get the protein I am...

11 December 2020 7,442 1 View

ChIP: why is my cell solution viscous after harvest?

I have performed a ChIP (on 15cm plates) by making a fresh PFA solution, adding it to a 1% final concentration to my cells for 10 minutes and neutralizing the PFA with 2.0M glycine added to a...

22 November 2020 681 2 View

Has anyone used the DSS crosslinker for ChIP?

I am hoping to crosslink the protein-protein interactions in cells as well as the protein-DNA interactions (for which I use formaldehyde). Has anyone tried using the crosslinker DSS for this purpose?

04 August 2020 1,993 2 View

Why aren't there spaces between the bands in my protein gel?

I ran a 5-15% gradient gel that I handmade and ran it overnight. I also left an empty lane between each sample and they still managed to spread into one another. I loaded 170 ug of sample (I know...

15 May 2019 8,868 5 View