I have a ABI 3500xL in my lab and I would like to know the opinion of someone who has already used this kit. Is the performance and quality comparable to the Thermo Scientifcs kit?
Hello everyone I have a model of an area of Lisbon designed on Envi-met but the top of the model is close to the top of the buildings. When I try to add cells to the top of the model the program...
10 June 2024 3,556 0 View
I am retiring, but still interested in academic work. How can I change the email to which you send notifications? Thanks.
12 May 2024 6,436 1 View
In a gene expression analysis since RQ usig delta Cq method depends on reaction efficiency do I'll have to make a standard curve every reaction?
21 January 2024 9,528 5 View
Dear Sir, On belfalf of the others Editors-in-chief of Jornal Brasileiro de Psiquiatria, we would like to know how can we update the page...
29 August 2023 2,118 1 View
I have been unable to find this article: Hensley, W. E., & Batty, P. (1974). The measurement of communication anxiety among students in public speaking courses. Indiana Speech Journal,...
11 July 2023 6,768 1 View
I'm new to ELISA assays and have heard that multiplexing can be difficult. In your experience, what are some of the challenges that come with multiplexing? Is detecting up to 3 targets doable?
21 June 2023 1,373 3 View
I have these squiggles that are appearing in my GAD67 IF stain and I'm not sure why. I've used this secondary antibody (Cy5 goat anti-mouse) with another primary antibody and never had this...
26 June 2022 2,095 9 View
I'm getting a lot of non-specific staining with a polyclonal ERa antibody I'm using. There's so much of it that I'm beginning to think that my incubation period (48 hrs at 4C) is too much....
06 March 2022 7,273 3 View
Can the rolling ball radius tool be used alone to reduce background in fluorescent images? Why or why not? If not, what are some additional methods to further remove background after rolling ball...
23 February 2022 3,896 3 View
I'm looking for alternatives to Minocycline that would be relatively safe to administer during pregnancy in a rodent model. So far, I've only found one paper that gives this drug prenatally and no...
04 November 2021 496 1 View
Hi All We have been using this instrument for about 6-7 yrs and this is the first time we have come across a raw data like the one given in the picture below. During the run there were no error...
14 March 2024 6,252 0 View
I have several patients with TBI/ABI #acquired brain injury I am reviewing data to assist in better strategic outcomes and am curious to the effects of Diabetes, epilepsy and sensory...
11 January 2024 4,152 0 View
Ineed a recipe.
13 December 2023 9,781 0 View
Dear all, I'm wondering about a possible substitution of a G>A base or a deletion of the G base in view of the chromatograms I've analyzed. I would like to point out that I have observed the...
21 November 2023 8,904 2 View
We are trying to calibrate our 7500 but we keep getting Error 8 when we do Background calibration. Any help will be appreciated. Thanks
13 November 2023 4,785 0 View
Hello everyone, I have a question that related to ABI Biosystem Stepone for detection whether virus exist or not in my cell culture sample. I should detect specific virus type in my cell culture...
29 August 2023 5,961 0 View
Hi ResearchGate community, I have been trying to learn more about the optical differences between block-based real-time PCR machines like ABI StepOne versus rotor-based machines such as MIC or...
22 July 2023 963 6 View
For your information, I am using ABI StepOne Plus machine. The housekeeping gene is Beta-actin. However, the results showed its melting curve and fluorescence plot look good. Appreciate it if...
13 November 2022 9,861 1 View
Regularly on the plasmid matrix, the following sequencegrams began to be obtained. In your experience, what could it be? Thanks!
21 February 2022 5,092 1 View
I have difficulty in converting my FASTA file into an ABI form in order to view the chromatogram of my sequence on Bioedit software. Anyone with a solution, please help.
15 February 2022 1,961 3 View