Can I use carbo-free blocking solution in place of normal goat serum?

I'm looking to try this in place of normal goat serum for a wisteria floribunda lectin stain. In my IF protocol, 10% of the final volume of my blocking solution is goat serum. If I were to go the...

07 February 2021 7,520 6 View

Can problems with blocking cause high background and weak antibody signal during immunofluorescence staining?

I'm working with 40um fresh frozen (non-perfused) sections of rat brain tissue and I'm staining for wisteria lection (immunofluorescence). After staining, my tissue has high background and a weak...

10 November 2020 3,620 7 View

Does anyone work with femoral and tibia radiography on rats or mices?

I would like to know what protocol followed for animals undergoing some treatment that induces some type of osteopenia / loss of bone density.

13 July 2019 1,731 2 View

What are the best research-based tools to teach pronunciation of L2 segments to second language learners?

Last year I attended an International Conference at the University of Utah about the teaching of pronunciation in second language learning and teaching. I notice that there was a general...

23 May 2018 5,599 2 View

How is your research likely to improve the approach to measuring performance quality?

I would be very interested in reading your publications related to measuring health and safety/ quality/ operations management performance - especially those related to recommendations for...

17 February 2017 7,501 2 View

Statistical Data: Experimental Design (teaching, how many students, control and normal group, definite conclusions)?

An innovative proposal, so say a new way of presenting contents or a small book with innovative ways of creating reasoning patterns: What do I need to have to prove to others, in a fully...

30 May 2016 9,386 3 View

Can you suggest a good assay to determine paracellular permeability in epithelial cells?

I am establishing a bovine oviductal epithelial cells culture using inserts of 0.4um pores and I am trying to get the cells polarized with this system. I would like to use the permeability assay...

16 January 2014 7,820 18 View

Does anyone know how test strips measure current for glucose detection?

Do they work by lateral flow and if not is it possible to make a lateral flow device with something like a test strip?

23 October 2013 7,767 4 View

How would you teach occupational safety and health management to social science classes compared to/contrasted with engineering classes?

I am planning to teach OSH to management students for the first time. I have had much experience teaching this field to technical students (engineering students, operators, etc.) and I am thinking...

01 January 1970 9,065 6 View

When doing research in a small island context, how can you show relevance to a more international/ developed nation audience?

Should papers be written from the context of a developing nation, rather than just an island? How can I show the relevance of my research to the USA, UK, the EU? Do I use my country's demographic...

01 January 1970 7,564 6 View

When to use Rox/No-Rox in qPCR instruments?

We know that passive reference dye/ROX can help reduce variations of fluorescent values in samples. When utilizing the Rotor Gene-Q for the real-time PCR experiment, usually, the SYBR Green...

29 August 2020 10,010 5 View

A decreased fluorescence RdRp (FAM) was found proportional to increased fluorescence N (JOE), is it abnormal?

01 May 2020 3,827 3 View

Why are microsatellite stutter peaks changing on repeated fragment analysis runs of same PCR product?

I've run a couple hundred samples using a microsatellite panel, and recently some of the runs show very exaggerated stutter peaks smaller than the true allele size. To troubleshoot the issue,...

01 April 2020 4,501 7 View

Can we substitute commercially available Sequencing buffer with self made buffer (400 mM Tris-HCL, 10mM MgCl2) ??

If anyone can suggest if I can use a lab made sequencing buffer of (400 mM Tris-HCL, 10mM MgCl2 and pH 9) in place commercial Sequecing buffer while performing Cyclic sequencing. How does the...

11 December 2019 9,649 3 View

How to mine the TCGA Database for RNA sequencing?

Anyone with advice on how to mine data from TCGA Database. I am interested in RNA seq data, i have a gene of interest i to analyze but i cannot seem to locate where to indicate the gene i am...

13 November 2019 5,262 3 View

How to mine the TCGA Database for RNA sequencing?

Anyone with advice on how to mine data from TCGA Database. I am interested in RNA seq data, i have a gene of interest i to analyze but i cannot seem to locate where to indicate the gene i am...

10 November 2019 2,362 3 View

Does Exosap needed 10 times dilution for cleaning for PCR product for sequencing?

Which manufactures/MNC'S EXOSAP needed 10 times dilution before use?

04 May 2019 5,558 1 View

Multi Locus Sequence typing?

Dear All, My Question is It ABI files are necessary for MLST analysis ? I lost My data regarding Forward Read sequences I can make its complementary sequences but one of my friend told me Abi /...

04 May 2019 2,849 3 View

How to validate methylation levels in HRM analysis?

Hello All, So, I am validating my Microarray results by HRM (High resolution melting) analysis. The CpG sites obtained by me are both hypo and hypermethylated. In the process of running my...

04 May 2019 5,949 3 View

How many samples by percent from total should be checked to validate that primers are working specifically and efficiency ?

Hello everyone, I hope you will understand what I want to ask. For exemple: I have 500 genomic DNA samples (different genotypes of same plant species) and I have 10 pairs of primers (Fwr+Rwr). I...

03 April 2019 4,147 6 View