The ABI chromatogram is the raw data you get from the sequencer. It shows all the ambiguities that possibly arise due to double peaks, missing peaks, compressions, etc. The FASTA file is the (usually computer-derived) most likely interpretation of such a chromatogram. It is therefore not possible to reconstitute the original chromatogram from the FASTA file, unless someone would like to generate a theoretically possible ABI chromatogram from a FASTA sequence file, but to what purpose ?

Maybe you could try to visualize and work on your fasta directly (for whatever goal you have in mind)?

If so, a tool like MEGA can help: https://www.megasoftware.net/

you can try on this below link,

online converter

http://www.dnabaser.com/download/Abi-to-Fasta-converter/abi-to-fasta-converter.html Aklilu Alemayehu