I have used an Ab CD56 (Roche) however, in breast in situ lesions, I have seen myoepithelial staining on my FFPE tissue as well as immune cells. Although this has been reported back in 1973-74 but not with this particular Ab, I was wondering has anybody also seen very similar pattern? The myoepithelial staining makes it very hard for us to ensure the stained immune cells are infact immune cells (NK cells). Does it seem the Ab is non-specific? It was not the dilution of Abs and any other technical IHC issues (ruled them out). I am looking forward to use a different CD5 Ab if possible.
Any suggestion?
Hi dear,
My advice is use CD44 and CD74 as breast cancer markers for both diagnosis and therapy.
Hi dear,
My advice is use CD44 and CD74 as breast cancer markers for both diagnosis and therapy.
Fucoidan-dependent Increased Membrane Components in HepG2 Cells: Effect of Fucoidan Is Not Due to Gene Expression
KOU HAYAKAWA1 and TAKEAKI NAGAMINE
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