Yes, Capto Q ImpRes resin has been used in various research and bioprocessing contexts, though not traditionally for plasmid purification from bacterial lysates. It's primarily designed for polishing steps in protein purification due to its high-resolution strong anion exchange (AEX) properties and rigid agarose base matrix, which enables high flow rates and pressure tolerance. However, it is possible to use it for plasmid DNA (pDNA) purification under certain conditions because plasmids are negatively charged and can bind to anion exchangers like Capto Q.

Hi, thank you very much for your response. I need to test it. Maybe because it is a strong anion exchanger, we will have some challenges in the final elution step of the plasmid.

Zarin Sharifnia Have you used Capto Q for plasmid Purification , if so , can I get the Protocol for the CaptoQ for plasmid purification if possible