Has anyone used Ambion® Cells-to-CT™ Kits for M17 neuroblastoma cells?

Fu Hung Shiu @Fu-Hung-Shiu

11 August 2015 1 6K Report

I am planning to do a real time RT-PCR BDNF gene expression for M17 cells.

I am wondering anyone has ever used Cells-to-CT™ Kits for M17 ( SK-N-BE(2) neuroblastoma cell line)

Or anyone can provide different kit for real time RT-PCR for bdnf gene expression for M17 cells?

Thanks!

Thomas

S. Béatrice Marianne Ewalds-Kvist

Dear Fu,

It seems to me that Amelia Compagni on RG could advise you.

Badges
Science topic

More Fu Hung Shiu's questions See All

Is expert panel indispensable for pedagogical framework & scale development?

Hello everyone, Thank you for your time and patience in advance! I attempt to develop a pedagogical framework and a competence scale for my PhD project. I have developed the drafts of both. I...

06 May 2024 2,239 3 View

What experiments should I design to investigate the role of exosomes in antigen presentation and the underlying mechanisms?

As a clinical medicine student investigating the role of exosomes in acute kidney injury, my research has progressed to the point where I need to validate the contributions of exosomal membrane...

15 April 2024 8,330 0 View

What experiments should I design to investigate the role of exosomes in antigen presentation and the underlying mechanisms?

As a clinical medicine student investigating the role of exosomes in acute kidney injury, my research has progressed to the point where I need to validate the contributions of exosomal membrane...

15 April 2024 2,628 0 View

Having both composite reliability and Cronbach's alpha above 0.6?

Composite reliability above 0.6, Cronbach's alpha above 0.6, and AVE above 0.5 are present simultaneously. Is this acceptable? Please share valid references to cite the same content." "Rho_c is...

06 March 2024 4,624 1 View

Where can I find a json dataset that matches my description?

Excuse me. I'm currently doing experiments on ray tracing in indoor environments. But I couldn't find a 2D data set for complex indoor environments. You know, the format of the data set that I'm...

29 February 2024 4,767 0 View

How to calculate the ore-forming temperature base on elemental composition of bulk grain sphalerite?

Dear Scientists and Colleagues, I trust this message finds you well. I am reaching out to seek your valuable expertise and guidance on a matter related to my current research. My focus revolves...

16 February 2024 8,137 2 View

Getting contacted by a potential PI?

Dear Scholars: Last year, I applied for a postdoc researcher job from any a number of labs in Australia but didn't have any reply from any one PI. I did research on these labs about works they...

26 January 2024 7,817 0 View

Why has another individual with the same name mistakenly claimed authorship of my article on ResearchGate?

Chun-Hung Chen*, Kuan-Wei Chen, Yu-Fan Chen, Chia-Yin Lin (2023), “The New Form Agency Problem: Cooperation and Circular Agency,” The B.E. Journal of Economic Analysis and Policy. 1-20. ISSN...

26 January 2024 435 4 View

How to pick up top 3 genes from the top genes in two different comparisons in DESeq analysis?

I have got my DESeq results back - heatmaps of top 30 genes for each comparison. - I have got top 30 genes from comparison group I, these 30 are highly expressed in condition A but not in...

18 January 2024 2,058 0 View

What is the difference in the quantity and reactivity of carboxylate groups in nanocellulose and sodium alginate?

What is the difference in the quantity and reactivity of carboxylate groups in carboxylated nanocellulose prepared by the tempo/NaClO/NaBr oxidation system compared to carboxylate groups in sodium...

25 December 2023 4,443 2 View

Which cell line will be the reference for human neuroblastoma?

We are implementing a grant related to the study of supertargets in secondary cultures of human neuroblastoma cells - IMR-32, Kelly, SH-SY5Y, SK-N-AS etc. Comparing gene expression between cell...

16 April 2024 7,706 2 View

Protein extraction from SH-SY5Y neuroblastoma cells and differentiated neurons?

I am working with SH-SY5Y cells and differentiating them into neurons using retinoic acid. I need to extract protein from them in order to conduct a western blot, however, I am having trouble...

31 January 2024 5,056 3 View

Can anyone identify what type of cell culture contamination this is?

Hello, Our lab is struggling with contamination despite taking every precaution necessary to maintain a sterile environment. The images shown are mature iPSC-derived neurons, but the...

03 October 2023 9,110 4 View

Any retinoic acid protocols for differentiating IMR32s?

Hi all, I am trying to differentiate my IMR32 neuroblastoma cells using retinoic acid or a similar treatment, and was wondering if anyone had any protocols you've had success with that you would...

10 August 2023 8,042 0 View

Contamination in sk-n-sh neuroblastoma cell culture?

Hi, I’m trying to culture sk-n-sh neuroblastoma cells. After 8th day of the culture cell morphology disappears and the accumulated structure appears (Please see the photographs). Could anyone make...

29 July 2023 4,952 0 View

¿What abtibodies can I use to evaluate oxidative stress in a cell line Using immunofluorescence ?

Hi, I want to evaluate oxidative stress in a neuroblastoma cell line, I know about SOD1, but I think it's not enough, so I was investigating and I found 4-hydroxynonenal, I have this in my lab...

27 June 2023 9,084 3 View

Which are the best conditions to transfect SK-N-SH with Nucleofector (Amaxa)?

We want to transfect SK-N-SH cells with Nucleofector (Amaxa), but our device don´t include the proper program to do it (X-005 or X-05). Does anybody know which are the settings corresponding to...

15 March 2023 499 3 View

How long would it take to differentiate SHSY5Y cells with retinoic acid?

Hi all, I am trying to differentiate SHSY5Y human neuroblastoma cells into neurons with the retinoic acid protocol. I add 10uM retinoic acid into 3% FBS containing DMEM for three days, and...

21 February 2023 8,474 4 View

Looking for human neuroblastoma cell lines from different stages of disease?

Hello everyone. I am looking for human neuroblastoma cell lines from different stages of disease. To clarify, i am looking for NB cell lines that are representing patients with different stages of...

25 January 2023 5,054 0 View

What is the most common normal neuronal cell lines ?

Dear all! now im working on my thesis (Anti Cancer Effect of Multi walled carbon Nanotubes ). im using SH-SY5Y neuroblastoma cell line and i need a normal neuronal cell line in order to test the...

21 December 2022 4,604 2 View