Hello there,
Has anyone transfected anf of these cell lines and can suggest a protocol? We need a cell line that expresses a certain gene to try our fluorescent construct on, so we don´t mind which type of cell line it is as long as it expresses the gene we need. We already checked that, they all express the gene, but now we would like to know which one should we try first, which one is easier to transfect and more efficient. If you could suggest protocols or good cell lines in this list that would be awesome.
- IMR-32 Human neuroblastoma cell line Neuroblast Brain.
- HEK 293 Human embrionic kidney cell line Epithelial Kidney.
- HT29 Human colon adenocarcinoma cell line Epithelial Colon
- A-549 Human adenocarcinomic alveolar basal epithelial cell line Epithelial Lung
- Hep G2 Human hepatocellular carcinoma cell line Epithelial Liver
- Hepa RG
- JURKAT Human T cell lymphoblast-like cell line Lymphoblast T lymphocyt
- A-431 Human epidermoid carcinoma cell line Epithelial Skin
- HCT116 Human colorectal carcinoma Epithelial Colon
Dear Beatriz María Fernández Santos,
I have used HEK 293 and Jurkat. I would recommend the HEK 293 cell line. It is widely used for expression of various genes.
For example, you could try googling "hek 293 lipofectamine".
Jurkat is a T-cell line and not adherent. Generally speaking, non-adherent cell lines are more difficult to transfect than adherent cell lines.
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