The best RNA isolation kit for lncRNA RNA-Seq?

Hi, I am preparing a new project on lncRNAs in human cancer cell lines. I scanned through many high-impacted journals to find the best RNA isolation kit that would also isolate small RNAs and that...

09 February 2021 4,180 5 View

What tools or software can I use in order to visualize EEG brain to brain synchrony?

I want to visualize brain to brain synchrony results (using EEG) as an illustration of two 3D heads with lines connecting the electrodes positions between the two heads where brain to brain...

16 November 2020 7,742 2 View

Can anyone please post a link to a reliable rat cDNA primer database?

I am looking for a source of cDNA primers, rat genome, real time PCR. Previously I used rtprimers bd, but is seems to be out of service for a while now.

01 September 2020 5,132 3 View

Silica gel in the sample on RP-HPLC ?

Hi Guys! Excuse my ignorance but I have little experience with RP-phases. I've managed to initially purify my peptide product using normal phase flash-chromatography. Unfortunately, Ihad to use a...

20 July 2020 7,274 14 View

Anyone knows what causes eye lid problem in Siberian hamster?

Several months ago we observed some strange changes around eyes of several individual Siberian hamsters (Phodopus sungorus) in our colony (outbred). I attach pictures. It looks like an...

17 July 2020 9,941 3 View

Is there any way to receive Hirsch index excluding self-citations from the Web of Science core collection?

The only thing I see is the Hirsch index value including all citations. Despite looking through the WoS tools I could not find any way to exclude it. However, I'm quite sure there must be some way...

15 June 2020 6,324 2 View

What is the scaling law for thermal damage to biomolecules/proteins from ultrashort heating?

It is well known that heating can denature proteins. However, what does happen to proteins in the case of short and ultrashort (microsecond or nanosecond scale) heating to extreme temperatures...

05 June 2020 6,650 1 View

Challenges in medical education in pandemic era?

These days many of academic teachers around the world have switched to e-teaching due to current epidemiological situation related to SARS-CoV-2. This is a great challenge to both students,...

31 March 2020 2,025 5 View

COVID-19 at psychiatric clinic?

In the current pandemic situation, it is not possible to completely avoid a psychiatric patient carrying COVID-19 or being directly symptomatic. In addition, medical priority is to help anyone....

19 March 2020 4,389 10 View

How to visualise cholesterol crystals inside tissue/cell?

Hi! I am searching for a method of visualisation/staining protocol for cholesterol crystals (not a free cholesterol, just crystalized) in cells as well as tissues? anyone has any experience??

04 March 2020 5,669 3 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

No title

02 March 2021 3,060 3 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View