Do you need to acidify your samples prior to desalting with C18?

I am wondering how important pH is here. I have a sample tryptic sample in 50mM TEAB, wondering if I need to add in TFA to ~2% before desalting. I understand the need to acidify samples with ion...

18 October 2020 6,639 3 View

Probelm with expressing hetrologous proteins using Pichia pastoris?

07 December 2019 7,502 6 View

How to calculate flat membrane pore size distribution using membrane DI flux?

Almost all membrane pore characterizing techniques cannot be used due to the mechanical instability of the flat membrane. I want to use deionized water flux (DI) and mathematical way to determine...

04 December 2019 3,850 2 View

Which part of polypeptide is susceptible for reaction?

A given polypeptide protein has side chains and terminal (N- and C-). For any modification or conjugation reaction which part involved in reactions (side chains or terminals). Thank you !!

17 October 2019 575 2 View

For many days enzyme expriment, fresh medium of enzyme need to be replaced regularly. At what time interval the medium should be replaced ?

For many days enzyme experiment, fresh medium of enzyme need to be replaced regularly. At what time interval the medium should be replaced by new to keep activity of enzymes?

01 September 2019 7,619 4 View

Can i used MTT to determine soil dehydrogenase?

In my understanding there are three main types of tetrazolium dyes that are used to determine dehydrogenase incluing MTT, INT, and TTC and for some reason i have encountered that MTT is rarely...

11 September 2018 3,590 2 View

Has anyone implementation of a distributed community detection algorithm?

Hi, I need urgently implemented code for a "distributed community detection algorithm" in java, python, matlab or .... Can anyone help me?

03 September 2018 4,469 3 View

Any idea why this cloning is not working?

Hi all, I am trying to clone a 750bp Age1/Xho1 fragment cut from a minipreped cDNA into another minipreped vector of 7.5Kbp (Amp) cut with the same enzymes. Insert and vector bands were cut and...

05 May 2018 9,946 12 View

What is the right journal to publish a survey paper in transportation?

What is the right journal to contact for publishing a survey paper in transportation?

05 March 2018 2,981 3 View

Oxygen Glucose Deprivation on mature oligodendrocytes cell lines not working?

27 December 2017 4,524 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View