Can Fecal Microbiota Transplantation from young heathy person to elderly persons improve health conditions?

06 January 2021 7,353 10 View

Bacteriophages very effective against infections. Some bacteriophages can be oncolytic and effective against cancers. How B_P recognize cancer cells?

Bacteriophages very effective against infections. Many of them such as anti-Staphylococcus or anti-Streptococcal widely used in practical medicine. Some bacteriophages can be oncolytic and...

03 January 2021 4,769 1 View

Is a new vaccines against traditional COVID-1 also effective against new, mutated strain of COVID-19?

I have question about efficacy of new vaccines against new, mutated strain of COVID-19. Is new vaccines against traditional COVID-1 also effective against new, mutated strain of COVID-19?

01 January 2021 9,737 5 View

Designing experiment of fluorescent titration?

I would be grateful if someone would advise or give a link on a detailed fluorescence titration technique. I am interested in setting up an experiment on binding of a receptor to a...

28 September 2020 5,641 7 View

Should be an Immunization for people who has had a COVID-19, convalescents, and some level of antibodies exist in their blood?

Could be a questionable. Before immunization to Virus SARS-CoV-2. Should be convalescents or people with antibodies to virus in blood still undergo immunization? Should be convalescents as part...

13 September 2020 7,147 7 View

Can you inject human exosomes to mice?

08 February 2020 7,507 3 View

Crosslinking of proteins in tissue lysate for co-Immunopercipitation?

Greetings, I am trying to detect transient protein-protein interactions in tissue lysates from mouse skeletal muscle. I have tried co-IP, and even though I am able to get a good IP of my...

10 November 2017 5,546 2 View

Does anyone have experience looking for correlations withing multi-variate time series sensor and GPS data?

Am starting to collect a small set of diabetes relevant (T1) time series data (movement, blood glucose, sleep, etc.) Have talked to many people who are doing such research, but few people seem to...

09 March 2017 2,254 3 View

Is the double slit experiment the mind game with Holy Spirit?

Gun G,Screen with two openings (the double slit) S,Impact screen I. Because of information conservation (cf. S.Hawking), the information about the events at S is recorded and is being spread...

29 September 2016 1,564 14 View

What conditions (pH, salts, etc.) are best suited for ssDNA adsorption on silica-spin columns?

In order to isolate single-strand DNA from M13-based phagemid, we intended to use silica-spin isolation instead of phenol/chloroform extraction for some reasons. Due to unavailability of...

03 April 2014 9,143 4 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View

How to dissolve cross-linked Amberlite IRC120 H, hydrogen form resin?

Hello all, I have used Amberlite IRC120 H, hydrogen form acidic cation-exchange resin for the ion-exchange of waterglass [sodium silicate solution -- 40% Sodium silicate (mol ratio > 3.2)...

21 February 2021 5,614 3 View

How to prepare electrolyte of NaClO4 salt for Na-ion battery? Any problem with this salt?

Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...

21 February 2021 8,132 1 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Is CXCR5 on T cells affected after a cell sorting?

I have tried to sort CD8+ CXCR5+ in the ARIA but the purity of my sample is about 50%. After sorting, I stain my samples to verify purity but it is low. So, I thought about "downregulation" or...

15 February 2021 8,034 7 View

Any way to separate out fluorescent channels from a composite image?

Hi there, I've been using the EVOS Auto II microscope for some time now and usually I will save the transmitted light/bright field image and the images from fluorescent channels as separate...

15 February 2021 546 3 View

Please, How can I plot a channel capacity of MIMO and SISO in matlab?

CSISO= f(frequency) : plot(f, CSISO) ? CMIMO= f(frequency) : plot (f, CMIMO) ?

13 February 2021 5,225 4 View