Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

Why am I getting such large heats of dilution in my Isothermal Titration Calorimetry Experiment?

I am trying to measure binding of calcium to an expressed protein we are studying and am getting very large heats of dilution for the calcium solution in my control tests. We prepared a 1 M CaCl2...

07 February 2021 7,532 1 View

I am looking to develop a basic oral microbiome assay. Can anyone advise on culture vessel, conditions and organisms that require minimal maintenance?

I am looking to investigate oral care products with an easy-to-prepare culture of orally derrived microorganisms.

21 January 2021 3,448 1 View

Cause of blisters in ALD layer at the edge of electrode?

I've encountered a defect during processing that I can't figure out, so I'm hoping I might find some ideas here. My mask design is a microelectrode array, with 60 microelectrodes with diameter...

19 January 2021 665 1 View

Difficult to silence gene?

I have been trying to silence a gene of interest but I could not get silencing efficiencies beyond 50% My method of detection is via RT-qPCR and samples were extracted 48hrs post transfection. Are...

09 January 2021 3,404 4 View

What form of data analysis should I use for my pilot study?

I am currently conducting my final year dissertation which is looking at the efficacy of common police lineup practices. However, before I launch my study, I have put out a pilot study which is...

08 January 2021 4,376 4 View

Is it normal to use Tris buffer when testing for bacteriostatic properties of isolated compounds?

Hello, to start off I attempting to test the bacteriostatic properties of certain compounds. I bought a kit that contains some of the equipment to perform this analysis, however, in the included...

02 January 2021 2,338 2 View

Cleaning SGE Micro Channel Device?

Hello all, I have a 3-port SGE microchannel splitter in my Thermo Trace 1310 GC connected to an Isolink 2 combustion interface. My microchannel device is clogged and my flow is significantly...

29 December 2020 7,002 1 View

Weird J-V curve of solar cell?

Recently I am getting low performance (PCE and FF) and weird J-V curves on Pb-Sn-based perovskite solar cells. I have been trying to solve this for a couple of weeks now. I will like any...

19 December 2020 9,336 4 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

How to improve the buffer capacity of sea water?

Hello everyone I would like to improve the buffer capacity of seawater (pH ~8) in order to dissolve as much CO2 as possible without dropping the pH below 7.5 (a range between 7 - 7.5 is...

17 February 2021 2,797 2 View

Room Occupancy Calculation ?

15 February 2021 1,997 6 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

How to increase bacterial concentration of DNA from Laser capture microdissection samples?

I used laser capture microdissection, selected specific bacteria presented in ethanol-fixed slides. And DNA was extracted by Qiamp mini kit. The problem right now is we have pretty low...

09 February 2021 3,908 2 View

Are there any fast-acting, long-term fixatives compatible with FISH?

I'm looking for a fixative that works quickly but you can safely store cells in for a longer period (i.e. a few hours). Most importantly it must be able to fix chromatin well and be compatible...

03 February 2021 7,659 7 View

Proteins not stacking/ entering in a native PAGE. Can anyone help?

Hi. I am trying to study a biotin modification of my target protein in a crude cell extract and am interested to determine how much of my protein in getting biotinylated. After biotinylating my...

15 January 2021 2,214 4 View

What buffer to use for biotinylation as alternative to PBS?

I'm looking to biotinylate a protein that precipitates in PBS. The recommended reconstitution buffer for the protein is urea/Tris buffer, pH 7,5 (7 M urea, 5 mM EDTA, 15 mM mercaptoethanol, 20 mM...

04 January 2021 7,681 5 View

What are the solvent requirement to study protein conformational changes via NMR?

I wanted to study the conformational changes taking place in gamma D crystallin (eye lens protein) at low pH via NMR. What would be the preferred solvent requirements for this particular protein...

31 December 2020 2,103 4 View