Has anyone tested an alternative to Biotin 14-dCTP fill in for Hi-C?

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Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

How to Interpret goodness-of-fit test results?

Hi, How do I interpret the p-values of the goodness-of-fit test results in ordinal logistic regression? I'm looking for literature in the internet but I couldn't find any. The tests that I need...

28 May 2024 6,554 2 View

How do I fix "Error in UseMethod" in R?

Hi, I am trying to do an Ordinal Logistic Regression (OLR) in R since this is the regression analysis that I need to use for my research. I followed the tutorial video I found in YouTube in...

19 May 2024 4,660 4 View

How Do I Increase the Cronbach's Alpha of My Survey Questionnaire?

Hi, I need help on how to increase the Cronbach's Alpha of my survey questionnaire. The survey questionnaire uses a 7-point likert scale and I had 12 respondents for its pilot testing. The...

16 May 2024 1,697 7 View

Straightforward way to correct for rater variables?

I want to measure correlations between human ratings of second language speech and a number of quantitative measures in the speech, such as articulation rate. I have collected speech rating data...

02 May 2024 6,400 0 View

Multiple comparisons in Tukey HSD test?

Hello, As part of my statistical analysis for my thesis, I have done a number of one-way MANOVAs to look at whether there are between-group differences on a number of different...

28 April 2024 5,767 2 View

What was the study's aim comparing gel separator serum and gel separator LIH plasma tubes and their key findings?

This question aims to understand the rationale behind the study, which likely sought to evaluate the comparability of gel separator serum and LIH plasma tubes in clinical chemistry and immunoassay...

09 April 2024 6,680 1 View

Challenges of child care arrangement among international students?

what are the challenges faced by international students on full time study concerning child care arrangement?

03 April 2024 5,556 0 View

Disease-drug database based on clinical data?

Dear all, I am seeking to build a resource linking diseases with bioactive compounds, and include source (plant species) and range information as well. To make sure that I am not reinventing the...

29 March 2024 9,997 0 View

Can I publish a study protocol (RCT) if I am unsure the research will go ahead due to funding?

I am wondering about publishing a protocol for an interventional, prospective trial, but I am unsure about funding the trial. On one hand, I believe publishing a study protocol generates interest,...

27 March 2024 2,077 1 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

How to merge two or more linkage maps?

I have three linkage maps that needs to be integrated to create a consensus map for QTL mapping.

25 July 2024 6,505 2 View

How do I avoid HEK293 cell lifting during biotinylation?

I’m doing cell-surface biotinylation with HEK293 cells and after all the washing and biotin incubation steps (washing with PBS ph8, neutralizing unreacted biotin with 40mM Tris ph8) many of my...

23 July 2024 4,740 3 View

Effectiveness and Implementation of CCUS in Oil Fields: Genuine Progress or Demonstrative Effort?

Many studies have been conducted on Carbon Capture, Utilization, and Storage (CCUS) in oil fields to increase the recovery factor. Do you believe these studies are genuinely useful, or are they...

24 June 2024 7,441 0 View

Does anyone have suggestions for this esterification?

I have a very polar alcohol (soluble in MeOH, H2O, partially in heated DMSO). And a long chain fatty acid that I would like to couple. I can't find a common solvent for them. I can form the...

24 June 2024 9,319 0 View

Method to prevent crosstalk fluorescene signal between 2 dye labeled microparticles?

My goal is to see two kinds of particles mixed well in an aggregated form. My experiment was as follows: 1. Prepared Microparticles 1 + EDC/NHS + RhoB, and Microparticles 2 + EDC/NHS + FITC. 2....

19 June 2024 2,680 1 View

Cheaper alternative to XSens mocap system for ergonomic data acquisition?

Hello all, I'm starting a project where we want to automate the video analysis of people working in different environments to produce ergonomic measures like hip flexion, shoulder extension, etc....

16 June 2024 1,843 1 View

Should there be a difference in biotin management of Alopecia if the patient is a chronic energy drink user?

We have bumped into this energy drink, Celsius, with 1000% of RDA. I am starting to collect some information about this, but there is paucity of information.

08 June 2024 4,733 0 View

Laser Capture Microdissection using Zeiss Palm microbeam instrument ..?

Dear Researchgate community. Is anyone doing laser capture microdissection using the Zeiss Palm microbeam instrument? If so, do you have an alternate (non-Zeiss) source for 500uL adhesive...

06 June 2024 5,117 2 View

Will this 3C Analysis work or are the fragments too small/close together?

I'm doing a 3C analysis shown in the picture linked and attached below with the Bait fragments labeled and the target fragments that will be tested for interaction with the bait labeled with...

02 June 2024 8,038 1 View