I want to isolate it from the animal so I can test its effect as a kairomone. I have tried "milking" them like a snake without success. Does anyone have a successful protocol or suggestions?
Saliva from aquatic Hemiptera can be obtained with electrical pulses applied at the insertion of the first pair of legs to the thorax using an electronic stimulator according it was described in the article:
AUREA DAN, M. H. PEREIRA, A. L. MELO, A. D. AZEVED and L. FREIRE-MAIA (1993) EFFECTS INDUCED BY SALIVA OF THE AQUATIC HEMIPTERAN BELOSTOlMA ANURUM ON THE ISOLATED GUINEA-PIG HEART, Comp. Biochem. Physiol. Vol. 106C, No. I, pp. 221-228.