20 November 2020 4 8K Report

This photo is from a reportedly healthy Long-Evans rat. Several of these animals have similar findings. No other sign of illness. Has anyone out there seen this?

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Does anyone know if there is a species of sand lance (Ammodytidae) in the Gulf of Mexico?

I'd like to know if any sand lances are found, specifically in the Northern Gulf of Mexico (Texas, Louisiana, Mississippi).

01 April 2024 498 0 View

Working with nominal data in Excel / Stat plus, Chi square?

We are analyzing the response of >400 respondents to a survey, with values of -1 (no) 0 (neutral), and +1 (yes). The data were collected to assess opinions on feeding practices at zoos. An...

17 October 2020 1,203 2 View

Why is my negative control serum broadly reactive in my ELISA, where my positive control serum is highly specific?

I am trying to establish an ELISA to measure reactivity of serum from infected patients towards various viral proteins. Currently, my set up is on a neutravidin coated plate. I add whole cell...

16 June 2020 3,074 0 View

Phenol:chloroform DNA extraction from Icelandic soils turned PURPLE - why?

Soil was incubated in 2% CTAB overnight at 37C. I spun the samples down and took the supernatant directly into phenol:chloroform:isoamyl alcohol 25:24:1. The Icelandic soils turned pink and...

09 September 2019 2,374 2 View

What are the best approaches for manipulating gene expression in specific cell types of birds in vivo?

I am a PhD student currently working with zebra finch. I think that the ability to manipulate the gene expression of specific cells within living zebra finch embryos would be useful for my...

19 January 2019 4,210 1 View

Can anyone share research on unexplained weight loss in adult Down syndrome patients?

My older brother has Down syndrome and has unexplained weight loss over the past year. Bloodwork is all fine. He's been drinking Ensure twice a day and that hasn't led to weight gain. He had an...

07 August 2018 7,224 1 View

When making SDS-PAGE gels I have bubble formation between the gel and plastic comb. Typically on the end of the gel. Is there a way to prevent this?

I usually lose one or two lanes as a result of the bubbling. Is this typical? Please see photos. I have no visible issues of leaking. I use fresh APS. I clean my plates rigorously. Thanks to...

24 April 2018 9,766 8 View

Staining clumping K562 cells to check for morphology changes - what to look at?

I'm growing K562 cells in RPMI media with 10% FBS or 10% FBEssence to check for any growth preference. Cells are growing at the same rate in both, but in FBEssence there is reproducable clumping...

14 April 2018 5,592 1 View

Bland-Altman with a linear plot line?

We conducted a projected in which two measurement methods were compared aganst each other. The first was done with MRI and distances were measured from a anatomical point. The second was a...

17 February 2018 154 2 View

Can anyone definitively answer the question: do fixed brain samples in PBS with sodium azide need to be refrigerated for long-term storage?

Can anyone definitively answer the question: do fixed brain samples in PBS with sodium azide need to be refrigerated for long-term storage?

02 June 2015 6,651 3 View

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Is there an English Translation of the Carl Moller text: ZUR VERGLEICHENDEN ANATOMIE DER SILURIDEN?

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10 August 2024 4,347 1 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

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Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

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The Curse of Evolution and Complexity?

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ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

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In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

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Do You Know Untreated TMJ Affects Your Brain And Entire Body?

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What can be the minimum number of animals (rats) to be used for a pilot/preliminary study on intradermal drug delivery?

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What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

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How to Post-fix Mice pup's brains?

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