Has anyone performed an AlphaScreen using Perkin Elmer beads and plates, but reading with a BioTek Synergy 2?

26 November 2012 1 617 Report

This data is from a plate containing only PBS and 5% BSA excitation at 680, emission at 520, 530, 540, and 590. Seems like a lot of background fluorescence.

Parag Mahanti

I am currently performing an alpha screen assay with perkin elmer plates and beads and a biotek synergy 2 and I seem to have signal in those ranges and negative controls that go down to 4000. My excitation is at 680. Emissions at 570 and 620.

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