Unusually Busy Raman Background?

Hello, I am observing a nearly periodic background spectrum for a wide variety of SERS substrates. I see this response (attached below) for bare Au films, Cu films, and Au nanoparticles on...

15 February 2021 9,922 5 View

What crossover operators can be used for a GA that finds suboptimal positions for a set of buildings?

I'm currently working on my undergraduate thesis where I develop a genetic algorithm that finds suboptimal 2D positions for a set of buildings. The solution representation is a vector of real...

11 January 2021 9,835 4 View

How did Rutherford and Thomson derive the equation for average deflection of a particle hitting an atom?

08 November 2020 9,046 2 View

Converting csv files with data for mapping rainfall on QGIS?

Hi, I am hoping to produce rainfall and temperature maps for a current project in QGIS. I have csv data downloaded which have been imported into QGIS. My aim is to be able to produce rainfall...

10 October 2020 4,930 5 View

How to control variables in a water hammer experiment?

I'm doing a high school experiment on how the impulse of water hammer is affected by pipe diameter, and I'm unsure of how to set up my experiment. My primary concern is safety and validity: - Is...

06 September 2020 1,252 3 View

Incorrect simulated conductance value compared to analytical solution using ANSYS Maxwell 2D?

31 May 2020 7,991 3 View

Reaction of hexamethyldisiloxane (HDMSO)+O2=SiO2+?

I'm giving a presentation on semiconductors and specifically the SiO2 insulating layer. The presentation is on kinetics so I kind of need to know the reaction. Problem is the papers I'm reading...

30 March 2020 8,643 3 View

Efficient preps for chunky human adipose tissue?

Hi all, I aim to isolate SVF from the tissue. However, we sometimes receive big chunks of tissue that is not through liposuction. I've been wondering is there a ingenious way to efficiently prep...

24 February 2020 6,104 1 View

Predicting the Age of home buyers - model based method?

All, I have a data-set which contains information on both the type of property an individual(s) purchased, along with some information on the individuals themselves. However, I am missing age...

19 February 2020 5,728 3 View

Are full-scale performance-based drama projects for adult EFL learners worthwhile?

Hello, I wonder if anyone would be kind enough to help with research for my dissertation concerning attitudes and approaches to full-scale stage or screen performance-based drama projects for...

27 January 2020 3,167 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View